The role of flow in bacterial biofilm morphology and wetting properties.

Biofilm Confocal laser scanning microscopy Flow-induced-morphology Image analysis Transport phenomena Wetting

Journal

Colloids and surfaces. B, Biointerfaces
ISSN: 1873-4367
Titre abrégé: Colloids Surf B Biointerfaces
Pays: Netherlands
ID NLM: 9315133

Informations de publication

Date de publication:
18 Apr 2020
Historique:
received: 04 12 2019
revised: 26 03 2020
accepted: 11 04 2020
pubmed: 11 5 2020
medline: 11 5 2020
entrez: 11 5 2020
Statut: aheadofprint

Résumé

Biofilms are bacterial communities embedded in an extracellular matrix, able to adhere to surfaces. Different experimental set-ups are widely used for in vitro biofilm cultivation; however, a well-defined comparison among different culture conditions, especially suited to interfacial characterization, is still lacking in the literature. The main objective of this work is to study the role of flow on biofilm formation, morphology and interfacial properties. Three different in vitro setups, corresponding to stagnant, shaking, and laminar flow conditions (custom-made flow cell), are used in this work to grow single strain biofilms of Pseudomonas fluorescens AR 11 on glass coupons. Results show that flow conditions significantly influenced biofilm formation kinetics, affecting mass transfer and cell attachment/detachment processes. Distinct morphological patterns are found under different flow regimes. Static contact angle data do not depend significantly on biofilm growth conditions in the parametric range investigated in this work.

Identifiants

DOI: 10.1016/j.colsurfb.2020.111047 PMID: 32388030
pubmed: 32388030
pii: S0927-7765(20)30277-0
doi: 10.1016/j.colsurfb.2020.111047
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

111047

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that there are no conflicts of interest.

Auteurs

Federica Recupido (F)

Division of Chemical Technology, School of Chemistry, Aristotle University of Thessaloniki, University Box 116, 54124, Thessaloniki, Greece; Department of Chemical, Materials and Industrial Production Engineering (DICMaPI), University of Naples, Federico II, Piazzale V. Tecchio 80, 80125, Naples, Italy.

Giuseppe Toscano (G)

Department of Chemical, Materials and Industrial Production Engineering (DICMaPI), University of Naples, Federico II, Piazzale V. Tecchio 80, 80125, Naples, Italy.

Rosarita Tatè (R)

Institute of Genetics and Biophysics: "A. Buzzati-Traverso" (IGB-CNR), Pietro Castellino 111, 80131, Naples, Italy.

Maria Petala (M)

Department of Civil Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.

Sergio Caserta (S)

Department of Chemical, Materials and Industrial Production Engineering (DICMaPI), University of Naples, Federico II, Piazzale V. Tecchio 80, 80125, Naples, Italy; CEINGE, Advanced Biotechnologies, 80145, Naples, Italy. Electronic address: sergio.caserta@unina.it.

Thodoris D Karapantsios (TD)

Division of Chemical Technology, School of Chemistry, Aristotle University of Thessaloniki, University Box 116, 54124, Thessaloniki, Greece. Electronic address: karapant@chem.auth.gr.

Stefano Guido (S)

Department of Chemical, Materials and Industrial Production Engineering (DICMaPI), University of Naples, Federico II, Piazzale V. Tecchio 80, 80125, Naples, Italy; CEINGE, Advanced Biotechnologies, 80145, Naples, Italy.

Classifications MeSH