Dynamic profile for the detection of anti-SARS-CoV-2 antibodies using four immunochromatographic assays.
Antibodies, Viral
/ analysis
Betacoronavirus
/ immunology
COVID-19
COVID-19 Testing
COVID-19 Vaccines
Clinical Laboratory Techniques
/ methods
Coronavirus Infections
/ diagnosis
Humans
Immunoassay
/ methods
Pandemics
Pneumonia, Viral
/ diagnosis
Real-Time Polymerase Chain Reaction
SARS-CoV-2
Sensitivity and Specificity
Antibody
COVID-19
Lateral flow assay
SARS-CoV-2
Journal
The Journal of infection
ISSN: 1532-2742
Titre abrégé: J Infect
Pays: England
ID NLM: 7908424
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
03
04
2020
accepted:
04
04
2020
pubmed:
12
5
2020
medline:
7
8
2020
entrez:
12
5
2020
Statut:
ppublish
Résumé
In order to fight the SARS-CoV-2 pandemic infection, there is a growing need and demand for diagnostic tools that are complementary and different from the RT-PCR currently in use. Multiple serological tests are or will be very soon available but need to be evaluated and validated. We have thus tested 4 immunochromatographic tests for the detection of antibodies to SARS-CoV-2. In addition, we assessed the kinetics of antibody appearance using these assays in 22 patients after they were tested positive by RT-PCR. We observed great heterogeneity in antibody detection post-symptom onset. The median antibody detection time was between 8 and 10 days according to the manufacturers. All the tests showed a sensitivity of 60 to 80% on day 10 and 100% on day 15. In addition, a single cross-reaction was observed with other human coronavirus infections. Thus, immunochromatographic tests for the detection of anti-SARS-CoV-2 antibodies may have their place for the diagnostic panel of COVID-19.
Identifiants
pubmed: 32389784
pii: S0163-4453(20)30244-9
doi: 10.1016/j.jinf.2020.04.033
pmc: PMC7204722
pii:
doi:
Substances chimiques
Antibodies, Viral
0
COVID-19 Vaccines
0
Covid-19 aAPC vaccine
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e6-e10Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020 The British Infection Association. Published by Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest All authors have no conflict of interest to declare
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