Collagen-An Important Fish Allergen for Improved Diagnosis.

Allergens Animals Collagen Food Hypersensitivity / diagnosis Humans Immunoglobulin E Parvalbumins
Allergy diagnosis Collagen Fish allergy IgE IgE cross-linking

Journal

The journal of allergy and clinical immunology. In practice
ISSN: 2213-2201
Titre abrégé: J Allergy Clin Immunol Pract
Pays: United States
ID NLM: 101597220

Informations de publication

Date de publication:
10 2020
Historique:
received: 22 02 2020
revised: 09 04 2020
accepted: 23 04 2020
pubmed: 12 5 2020
medline: 15 5 2021
entrez: 12 5 2020
Statut: ppublish

Résumé

Fish collagen is widely used in medicine, cosmetics, and the food industry. However, its clinical relevance as an allergen is not fully appreciated. This is likely due to collagen insolubility in neutral aqueous solutions, leading to low abundance in commercially available in vitro and skin prick tests for fish allergy. To investigate the relevance of fish collagen as an allergen in a large patient population (n = 101). Acid-soluble collagen type I was extracted from muscle and skin of Atlantic salmon, barramundi, and yellowfin tuna. IgE binding to collagen was analyzed by ELISA for 101 fish-allergic patients. Collagen-sensitized patients' sera were tested for IgE binding to parvalbumin from the same fish species. IgE cross-linking was analyzed by rat basophil leukemia assay and basophil activation test. Protein identities were confirmed by mass spectrometry. Purified fish collagen contained type I α1 and α2 chains and their multimers. Twenty-one of 101 patients (21%) were sensitized to collagen. Eight collagen-sensitized patients demonstrated absence of parvalbumin-specific IgE to some fish species. Collagen induced functional IgE cross-linking, as shown by rat basophil leukemia assay performed using 6 patients' sera, and basophil activation test using fresh blood from 1 patient. Collagen type I α chains from barramundi and Atlantic salmon were registered at www.allergen.org as Lat c 6 and Sal s 6, respectively. IgE sensitization and IgE cross-linking capacity of fish collagen were demonstrated in fish-allergic patients. Inclusion of relevant collagen allergens in routine diagnosis is indicated to improve the capacity to accurately diagnose fish allergy.

Sections du résumé

BACKGROUND
Fish collagen is widely used in medicine, cosmetics, and the food industry. However, its clinical relevance as an allergen is not fully appreciated. This is likely due to collagen insolubility in neutral aqueous solutions, leading to low abundance in commercially available in vitro and skin prick tests for fish allergy.
OBJECTIVE
To investigate the relevance of fish collagen as an allergen in a large patient population (n = 101).
METHODS
Acid-soluble collagen type I was extracted from muscle and skin of Atlantic salmon, barramundi, and yellowfin tuna. IgE binding to collagen was analyzed by ELISA for 101 fish-allergic patients. Collagen-sensitized patients' sera were tested for IgE binding to parvalbumin from the same fish species. IgE cross-linking was analyzed by rat basophil leukemia assay and basophil activation test. Protein identities were confirmed by mass spectrometry.
RESULTS
Purified fish collagen contained type I α1 and α2 chains and their multimers. Twenty-one of 101 patients (21%) were sensitized to collagen. Eight collagen-sensitized patients demonstrated absence of parvalbumin-specific IgE to some fish species. Collagen induced functional IgE cross-linking, as shown by rat basophil leukemia assay performed using 6 patients' sera, and basophil activation test using fresh blood from 1 patient. Collagen type I α chains from barramundi and Atlantic salmon were registered at www.allergen.org as Lat c 6 and Sal s 6, respectively.
CONCLUSIONS
IgE sensitization and IgE cross-linking capacity of fish collagen were demonstrated in fish-allergic patients. Inclusion of relevant collagen allergens in routine diagnosis is indicated to improve the capacity to accurately diagnose fish allergy.

Identifiants

DOI: 10.1016/j.jaip.2020.04.063 PMID: 32389794
pubmed: 32389794
pii: S2213-2198(20)30423-2
doi: 10.1016/j.jaip.2020.04.063
pii:
doi:

Substances chimiques

Allergens 0
Parvalbumins 0
Immunoglobulin E 37341-29-0
Collagen 9007-34-5

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3084-3092.e10

Informations de copyright

Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.

Auteurs

Tanja Kalic (T)

Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.

Sandip D Kamath (SD)

College of Public Health, Medical and Veterinary Sciences, Australian Institute of Tropical Health and Medicine, Molecular Allergy Research Laboratory, James Cook University, Townsville, QLD, Australia; Centre for Food and Allergy Research, Murdoch Children's Research Institute, Melbourne, VIC, Australia.

Thimo Ruethers (T)

College of Public Health, Medical and Veterinary Sciences, Australian Institute of Tropical Health and Medicine, Molecular Allergy Research Laboratory, James Cook University, Townsville, QLD, Australia; Centre for Food and Allergy Research, Murdoch Children's Research Institute, Melbourne, VIC, Australia.

Aya C Taki (AC)

College of Public Health, Medical and Veterinary Sciences, Australian Institute of Tropical Health and Medicine, Molecular Allergy Research Laboratory, James Cook University, Townsville, QLD, Australia; Centre for Food and Allergy Research, Murdoch Children's Research Institute, Melbourne, VIC, Australia; Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Melbourne, VIC, Australia.

Roni Nugraha (R)

College of Public Health, Medical and Veterinary Sciences, Australian Institute of Tropical Health and Medicine, Molecular Allergy Research Laboratory, James Cook University, Townsville, QLD, Australia; Department of Aquatic Product Technology, Bogor Agricultural University, Bogor, Jawa Barat, Indonesia.

Thu T K Le (TTK)

College of Public Health, Medical and Veterinary Sciences, Australian Institute of Tropical Health and Medicine, Molecular Allergy Research Laboratory, James Cook University, Townsville, QLD, Australia.

Piotr Humeniuk (P)

Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.

Nicholas A Williamson (NA)

Bio21 Mass Spectrometry and Proteomics Facility, The Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC, Australia.

Diamond Hira (D)

Allergy Centre, Townsville, QLD, Australia.

Jennifer M Rolland (JM)

Department of Immunology and Pathology, Monash University, Melbourne, VIC, Australia; Department of Allergy, Immunology and Respiratory Medicine, Central Clinical School, Monash University and The Alfred Hospital, Melbourne, VIC, Australia.

Robyn E O'Hehir (RE)

Department of Immunology and Pathology, Monash University, Melbourne, VIC, Australia; Department of Allergy, Immunology and Respiratory Medicine, Central Clinical School, Monash University and The Alfred Hospital, Melbourne, VIC, Australia.

Danyi Dai (D)

Department of Allergy and Immunology, Children's Hospital at Westmead, Sydney, NSW, Australia.

Dianne E Campbell (DE)

Centre for Food and Allergy Research, Murdoch Children's Research Institute, Melbourne, VIC, Australia; Department of Allergy and Immunology, Children's Hospital at Westmead, Sydney, NSW, Australia.

Heimo Breiteneder (H)

Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.

Andreas L Lopata (AL)

College of Public Health, Medical and Veterinary Sciences, Australian Institute of Tropical Health and Medicine, Molecular Allergy Research Laboratory, James Cook University, Townsville, QLD, Australia; Centre for Food and Allergy Research, Murdoch Children's Research Institute, Melbourne, VIC, Australia. Electronic address: andreas.lopata@jcu.edu.au.

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Classifications MeSH

questionsmedicales.fr › Facteurs biologiques › Antigènes › Allergènes › Collagen-An Important Fish Allergen for...
questionsmedicales.fr › Eucaryotes › Animaux › Collagen-An Important Fish Allergen for...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Scléroprotéines › Protéines de la matrice extracellulaire › Collagène › Collagen-An Important Fish Allergen for...
questionsmedicales.fr › Maladies du système immunitaire › Hypersensibilité › Hypersensibilité immédiate › Hypersensibilité alimentaire › Collagen-An Important Fish Allergen for...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Collagen-An Important Fish Allergen for...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Isotypes des immunoglobulines › Immunoglobuline E › Collagen-An Important Fish Allergen for...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines contractiles › Protéines du muscle › Parvalbumines › Collagen-An Important Fish Allergen for...