Activation and functional modulation of satellite glial cells by oxaliplatin lead to hyperexcitability of sensory neurons in vitro.
Animals
Antineoplastic Agents
/ pharmacology
Connexin 43
/ metabolism
Ganglia, Spinal
/ drug effects
Glial Fibrillary Acidic Protein
/ metabolism
Neuroglia
/ drug effects
Neurons
/ drug effects
Oxaliplatin
/ metabolism
Potassium Channels, Inwardly Rectifying
/ metabolism
Satellite Cells, Perineuronal
/ metabolism
Sensory Receptor Cells
/ drug effects
Chemotherapy
Cytokines
Dorsal root ganglion
Neuropathic pain
Oxaliplatin
Satellite glial cells
Journal
Molecular and cellular neurosciences
ISSN: 1095-9327
Titre abrégé: Mol Cell Neurosci
Pays: United States
ID NLM: 9100095
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
12
12
2019
revised:
28
03
2020
accepted:
03
05
2020
pubmed:
12
5
2020
medline:
4
3
2021
entrez:
12
5
2020
Statut:
ppublish
Résumé
Platinum-based chemotherapeutics still play an important role in cancer therapy, however, severe side effects, such as painful neuropathy, occur frequently. The pathophysiologic mechanisms depend on the applied chemotherapeutic agent and are still controversial. In addition to neuronal damage, disturbance of glial cell activity may contribute to neurotoxicity. Here, we focused on the effect of oxaliplatin on satellite glial cell (SGC) function and on the activity of the dorsal root ganglion (DRG) neurons. SGCs were isolated as high-purity cultures and treated with 1 and 10 μM oxaliplatin for 2, 4 and 24 h. Subsequently, glial fibrillary acid protein (GFAP), reactive oxygen species (ROS), Connexin-43 (Cx-43), and inward rectifier potassium channel 4.1 (K
Identifiants
pubmed: 32389805
pii: S1044-7431(20)30122-6
doi: 10.1016/j.mcn.2020.103499
pii:
doi:
Substances chimiques
Antineoplastic Agents
0
Connexin 43
0
Glial Fibrillary Acidic Protein
0
Potassium Channels, Inwardly Rectifying
0
Oxaliplatin
04ZR38536J
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
103499Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare no conflicts of interest.