A rat arteriovenous graft model using decellularized vein.
Animals
Aorta
/ surgery
Arteriovenous Shunt, Surgical
/ adverse effects
Endothelial Progenitor Cells
/ metabolism
Hematopoietic Stem Cells
/ metabolism
Jugular Veins
/ metabolism
Macrophages
/ metabolism
Male
Rats, Wistar
Time Factors
Vascular Patency
Vascular Remodeling
Vena Cava, Inferior
/ surgery
Arteriovenous graft
CD90
decellularization
endothelial progenitor cell
macrophage
smooth muscle cell
Journal
Vascular
ISSN: 1708-539X
Titre abrégé: Vascular
Pays: England
ID NLM: 101196722
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
pubmed:
12
5
2020
medline:
22
9
2020
entrez:
12
5
2020
Statut:
ppublish
Résumé
The high rate of clinical failure of prosthetic arteriovenous grafts continues to suggest the need for novel tissue-engineered vascular grafts. We tested the hypothesis that the decellularized rat jugular vein could be successfully used as a conduit and that it would support reendothelialization as well as adaptation to the arterial environment. Autologous (control) or heterologous decellularized jugular vein (1 cm length, 1 mm diameter) was sewn between the inferior vena cava and aorta as an arteriovenous graft in Wistar rats. Rats were sacrificed on postoperative day 21 for examination. All rats survived, and grafts had 100% patency in both the control and decellularized groups. Both control and decellularized jugular vein grafts showed similar rates of reendothelialization, smooth muscle cell deposition, macrophage infiltration, and cell turnover. The outflow veins distal to the grafts showed similar adaptation to the arteriovenous flow. Both CD34, CD90 and nestin positive cells, as well as M1-type and M2-type macrophages accumulated around the graft. This model shows that decellularized vein can be successfully used as an arteriovenous graft between the rat aorta and the inferior vena cava. Several types of cells, including progenitor cells and macrophages, are present in the host response to these grafts in this model. This model can be used to test the application of arteriovenous grafts before conducting large animal experiments.
Sections du résumé
BACKGROUND
BACKGROUND
The high rate of clinical failure of prosthetic arteriovenous grafts continues to suggest the need for novel tissue-engineered vascular grafts. We tested the hypothesis that the decellularized rat jugular vein could be successfully used as a conduit and that it would support reendothelialization as well as adaptation to the arterial environment.
MATERIALS AND METHODS
METHODS
Autologous (control) or heterologous decellularized jugular vein (1 cm length, 1 mm diameter) was sewn between the inferior vena cava and aorta as an arteriovenous graft in Wistar rats. Rats were sacrificed on postoperative day 21 for examination.
RESULTS
RESULTS
All rats survived, and grafts had 100% patency in both the control and decellularized groups. Both control and decellularized jugular vein grafts showed similar rates of reendothelialization, smooth muscle cell deposition, macrophage infiltration, and cell turnover. The outflow veins distal to the grafts showed similar adaptation to the arteriovenous flow. Both CD34, CD90 and nestin positive cells, as well as M1-type and M2-type macrophages accumulated around the graft.
CONCLUSIONS
CONCLUSIONS
This model shows that decellularized vein can be successfully used as an arteriovenous graft between the rat aorta and the inferior vena cava. Several types of cells, including progenitor cells and macrophages, are present in the host response to these grafts in this model. This model can be used to test the application of arteriovenous grafts before conducting large animal experiments.
Identifiants
pubmed: 32390561
doi: 10.1177/1708538120923191
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM