Off-Chip-Controlled Droplet-on-Demand Method for Precise Sample Handling.
Journal
ACS omega
ISSN: 2470-1343
Titre abrégé: ACS Omega
Pays: United States
ID NLM: 101691658
Informations de publication
Date de publication:
05 May 2020
05 May 2020
Historique:
received:
14
11
2019
accepted:
09
03
2020
entrez:
12
5
2020
pubmed:
12
5
2020
medline:
12
5
2020
Statut:
epublish
Résumé
We present a simple, stable, and highly reproducible off-chip-controlled method for generating droplets-on-demand. To induce the droplet generation, externally pre-programmed positive pressure pulses are applied to the dispersed phase input while the continuous phase channel remains at constant input pressure. By controlling solely one fluid phase, the method allows for connecting multiple independent dispersed-phase channels to a single continuous channel. Experimental results show that the method allows for a droplet generation frequency of 33 Hz and a high reproducibility of droplets with standard deviations less than 5% of the mean value. Moreover, utilization of the off-chip-controlled method results in the simplicity in chip design and allows rapid (∼5 min) and cost-efficient (0.5 USD) prototyping of the device.
Identifiants
pubmed: 32391454
doi: 10.1021/acsomega.9b03883
pmc: PMC7203690
doi:
Types de publication
Journal Article
Langues
eng
Pagination
9684-9689Informations de copyright
Copyright © 2020 American Chemical Society.
Déclaration de conflit d'intérêts
The authors declare no competing financial interest.
Références
Lab Chip. 2013 May 21;13(10):1892-901
pubmed: 23567746
Anal Chem. 2017 Apr 18;89(8):4387-4391
pubmed: 28192966
Lab Chip. 2009 Jul 7;9(13):1850-8
pubmed: 19532959
Angew Chem Int Ed Engl. 2006 Nov 13;45(44):7336-56
pubmed: 17086584
J Microbiol Methods. 2005 Aug;62(2):181-97
pubmed: 16009276
Lab Chip. 2013 Sep 21;13(18):3689-97
pubmed: 23868204
Lab Chip. 2019 Jan 29;19(3):493-502
pubmed: 30623951
Lab Chip. 2009 May 21;9(10):1340-3
pubmed: 19417898
Anal Chem. 2016 Jan 5;88(1):339-53
pubmed: 26558892
Lab Chip. 2006 Mar;6(3):437-46
pubmed: 16511628
Cell. 2015 Sep 10;162(6):1309-21
pubmed: 26343579
Chem Soc Rev. 2010 Mar;39(3):974-84
pubmed: 20179819