The neuronal transcription factor Myt1L interacts via a conserved motif with the PAH1 domain of Sin3 to recruit the Sin3L/Rpd3L histone deacetylase complex.
histone deacetylases
neuronal differentiation
repressor-corepressor interaction
solution NMR spectroscopy
structure-function analysis
transcriptional repression
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
08
04
2020
revised:
27
04
2020
accepted:
28
04
2020
pubmed:
12
5
2020
medline:
8
5
2021
entrez:
12
5
2020
Statut:
ppublish
Résumé
The Sin3L/Rpd3L histone deacetylase (HDAC) complex is one of six major HDAC complexes in the nucleus, and its recruitment by promoter-bound transcription factors is an important step in many gene transcription regulatory pathways. Here, we investigate how the Myt1L zinc finger transcription factor, important for neuronal differentiation and the maintenance of neuronal identity, recruits this complex at the molecular level. We show that Myt1L, through a highly conserved segment shared with its paralogs, interacts directly and specifically with the Sin3 PAH1 domain, binding principally to the canonical hydrophobic cleft found in paired amphipathic helix domain (PAH) domains. Our findings are relevant not only for other members of the Myt family but also for enhancing our understanding of the rules of protein-protein interactions involving Sin3 PAH domains.
Identifiants
pubmed: 32391601
doi: 10.1002/1873-3468.13811
pmc: PMC8224932
mid: NIHMS1710064
doi:
Substances chimiques
MYT1L protein, human
0
Nerve Tissue Proteins
0
SIN3A transcription factor
0
Transcription Factors
0
Histone Deacetylase 1
EC 3.5.1.98
Sin3 Histone Deacetylase and Corepressor Complex
EC 3.5.1.98
Types de publication
Letter
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2322-2330Subventions
Organisme : NIH HHS
ID : S10 OD012016
Pays : United States
Informations de copyright
© 2020 Federation of European Biochemical Societies.
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