Visualizing Genome Reorganization Using 3D DNA FISH.

Cell Nucleus / genetics Chromatin / genetics DNA / genetics Genome / genetics Humans Imaging, Three-Dimensional In Situ Hybridization, Fluorescence / methods
Chromatin DNA FISH Gene regulation Genetics Nuclear organization

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 13 5 2020
pubmed: 13 5 2020
medline: 10 3 2021
Statut: ppublish

Résumé

Understanding how the genome is organized within the cell nucleus is increasingly recognized to be important to understand gene regulation. In 3D DNA fluorescence in situ hybridization (3D DNA FISH) labeled probes complementary to specific loci of interest are hybridized to the genome. The samples are then imaged using fluorescence microscopy, collecting z-stacks through the nuclei, and the relative positions of the hybridized probes are analyzed in the reconstructed 3D images. In this way 3D DNA FISH provides a powerful tool to interrogate how the organization of specific genomic loci changes in response to stimuli. This chapter describes protocols which have allowed us to produce consistent data in cultured cells and paraffin-embedded tissue sections.

Identifiants

DOI: 10.1007/978-1-0716-0623-0_5 PMID: 32394376
pubmed: 32394376
doi: 10.1007/978-1-0716-0623-0_5
doi:

Substances chimiques

Chromatin 0
DNA 9007-49-2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

85-95

Références

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BACPAC Children’s Hospital Oakland Research Institute. https://bacpacresources.org/

Auteurs

Alasdair Jubb (A)

Division of Anaesthesia, Department of Medicine, University of Cambridge, Cambridge, UK. awj23@cam.ac.uk.
CRUK Cambridge Institute, University of Cambridge, Cambridge, UK. awj23@cam.ac.uk.

Shelagh Boyle (S)

MRC Human Genetics Unit, MRC Institute of Genetics & Molecular Medicine, University of Edinburgh, Edinburgh, UK.

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Classifications MeSH

questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Noyau de la cellule › Visualizing Genome Reorganization Using 3D DNA FISH.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Structures de chromosome › Chromatine › Visualizing Genome Reorganization Using 3D DNA FISH.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Visualizing Genome Reorganization Using 3D DNA FISH.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Visualizing Genome Reorganization Using 3D DNA FISH.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Visualizing Genome Reorganization Using 3D DNA FISH.
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Traitement d'image par ordinateur › Imagerie tridimensionnelle › Visualizing Genome Reorganization Using 3D DNA FISH.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Hybridation in situ › Hybridation fluorescente in situ › Visualizing Genome Reorganization Using 3D DNA FISH.