Evaluation of inhibitor of apoptosis genes as targets for RNAi-mediated control of insect pests.
RNA interference
Tribolium castaneum
iap
red flour beetle
survivin
Journal
Archives of insect biochemistry and physiology
ISSN: 1520-6327
Titre abrégé: Arch Insect Biochem Physiol
Pays: United States
ID NLM: 8501752
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
18
12
2019
revised:
10
04
2020
accepted:
21
04
2020
pubmed:
13
5
2020
medline:
30
9
2020
entrez:
13
5
2020
Statut:
ppublish
Résumé
Apoptosis has been widely studied from mammals to insects. Inhibitor of apoptosis (IAP) protein is a negative regulator of apoptosis. Recent studies suggest that iap genes could be excellent targets for RNA interference (RNAi)-mediated control of insect pests. However, not much is known about iap genes in one of the well-known insect model species, Tribolium castaneum. The orthologues of five iap genes were identified in T. castaneum by searching its genome at NCBI (https://www.ncbi.nlm.nih.gov/) and UniProt (https://www.uniprot.org/) databases using Drosophila melanogaster and Aedes aegypti IAP protein sequences as queries. RNAi assays were performed in T. castaneum cell line (TcA) and larvae. The knockdown of iap1 gene induced a distinct apoptotic phenotype in TcA cells and induced 91% mortality in T. castaneum larvae. Whereas, knockdown of iap5 resulted in a decrease in cell proliferation in TcA cells and developmental defects in T. castaneum larvae which led to 100% mortality. Knockdown of the other three iap genes identified did not cause a significant effect on cells or insects. These data increase our understanding of iap genes in insects and provide opportunities for developing iap1 and iap5 as targets for RNAi-based insect pest control.
Identifiants
pubmed: 32394607
doi: 10.1002/arch.21689
pmc: PMC9945918
mid: NIHMS1870353
doi:
Substances chimiques
Insect Proteins
0
Baculoviral IAP Repeat-Containing 3 Protein
EC 2.3.2.27
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e21689Subventions
Organisme : National Institute of Food and Agriculture
ID : 2353057000
Organisme : NIGMS NIH HHS
ID : R01 GM070559
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI131427
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI163561
Pays : United States
Organisme : NIH HHS
ID : GM070559-14
Pays : United States
Organisme : NIH HHS
ID : 1R21AI131427-01
Pays : United States
Organisme : National Science Foundation
ID : IIP-1821936
Informations de copyright
© 2020 Wiley Periodicals LLC.
Références
Cell. 1994 Mar 11;76(5):777-9
pubmed: 8124715
J Cell Sci. 2001 May;114(Pt 10):1821-7
pubmed: 11329368
Dev Growth Differ. 2011 Feb;53(2):125-36
pubmed: 21338339
Methods Mol Biol. 2009;555:77-86
pubmed: 19495689
Mol Cell. 2008 Apr 25;30(2):123-35
pubmed: 18439892
Cell Death Differ. 1998 Jul;5(7):569-76
pubmed: 10200511
Sci Rep. 2017 Aug 21;7(1):8913
pubmed: 28827780
Cancer Biother Radiopharm. 2008 Dec;23(6):727-34
pubmed: 19111044
Proc Natl Acad Sci U S A. 2005 Aug 9;102(32):11337-42
pubmed: 16076951
J Virol. 1994 Apr;68(4):2521-8
pubmed: 8139034
Proc Natl Acad Sci U S A. 2018 Aug 14;115(33):8334-8339
pubmed: 30061410
Sci Rep. 2019 Jun 19;9(1):8775
pubmed: 31217512
Biotechniques. 2008 Apr;44(5):613-6
pubmed: 18474035
J Insect Physiol. 2010 Mar;56(3):227-35
pubmed: 19837076
Insect Biochem Mol Biol. 2016 Nov;78:78-88
pubmed: 27687845
J Med Entomol. 2016 Mar;53(2):304-14
pubmed: 26659858
Virus Res. 2003 Mar;92(1):107-11
pubmed: 12606082
Int J Mol Sci. 2018 Apr 04;19(4):
pubmed: 29617308
Mol Biol Rep. 2012 Feb;39(2):1691-9
pubmed: 21633890
Cell. 1995 Dec 29;83(7):1253-62
pubmed: 8548811
Chem Biol Drug Des. 2013 Sep;82(3):243-51
pubmed: 23790005
Curr Opin Mol Ther. 2006 Apr;8(2):115-21
pubmed: 16610763
Mech Dev. 2008 Mar-Apr;125(3-4):299-313
pubmed: 18083350
Annu Rev Entomol. 2020 Jan 7;65:293-311
pubmed: 31610134
Nature. 1996 Jan 25;379(6563):349-53
pubmed: 8552191
Sci Rep. 2018 Feb 27;8(1):3720
pubmed: 29487333
Toxicol Pathol. 2007 Jun;35(4):495-516
pubmed: 17562483
Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13786-90
pubmed: 8943013
Proc Natl Acad Sci U S A. 2010 May 11;107(19):8557-62
pubmed: 20410462
Curr Opin Insect Sci. 2014 Dec;6:1-8
pubmed: 26705514
Nature. 2008 Apr 24;452(7190):949-55
pubmed: 18362917
Methods Mol Biol. 2009;555:v-vi
pubmed: 19725188
Biotechniques. 2006 Apr;Suppl:4-5
pubmed: 16629381
Nature. 1998 Feb 19;391(6669):806-11
pubmed: 9486653
Sci Rep. 2017 Nov 9;7(1):15147
pubmed: 29123201
Genome Biol. 2008 Jan 17;9(1):R10
pubmed: 18201385
Nat Rev Cancer. 2003 Jan;3(1):46-54
pubmed: 12509766
Sci Rep. 2018 Feb 1;8(1):2061
pubmed: 29391456
J Virol. 1993 Apr;67(4):2168-74
pubmed: 8445726
J Biol Chem. 2002 Jun 28;277(26):23103-6
pubmed: 12011068
Insect Biochem Mol Biol. 2016 Sep;76:70-83
pubmed: 27418459
Genes (Basel). 2012 Nov 06;3(4):702-41
pubmed: 24705082
Mech Dev. 2006 Jul;123(7):530-47
pubmed: 16829058
Genome Biol. 2001;2(7):REVIEWS3009
pubmed: 11516343