Bone tissue engineering using adipose-derived stem cells and endothelial cells: Effects of the cell ratio.
Adipose Tissue
/ cytology
Alkaline Phosphatase
/ genetics
Apoptosis
Bone and Bones
/ physiology
Cell Differentiation
Cell Proliferation
Cells, Cultured
Collagen
Core Binding Factor Alpha 1 Subunit
/ genetics
Drug Combinations
Human Umbilical Vein Endothelial Cells
/ cytology
Humans
Laminin
Neovascularization, Physiologic
Osteogenesis
Proteoglycans
Stem Cells
/ cytology
Tissue Engineering
ADSC
HUVEC
angiogenesis
bone tissue engineering
osteogenesis
Journal
Journal of cellular and molecular medicine
ISSN: 1582-4934
Titre abrégé: J Cell Mol Med
Pays: England
ID NLM: 101083777
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
09
02
2020
revised:
06
04
2020
accepted:
22
04
2020
pubmed:
13
5
2020
medline:
30
4
2021
entrez:
13
5
2020
Statut:
ppublish
Résumé
The microvascular endothelial network is essential for bone formation and regeneration. In this context, endothelial cells not only support vascularization but also influence bone physiology via cell contact-dependent mechanisms. In order to improve vascularization and osteogenesis in tissue engineering applications, several strategies have been developed. One promising approach is the coapplication of endothelial and adipose derived stem cells (ADSCs). In this study, we aimed at investigating the best ratio of human umbilical vein endothelial cells (HUVECs) and osteogenic differentiated ADSCs with regard to proliferation, apoptosis, osteogenesis and angiogenesis. For this purpose, cocultures of ADSCs and HUVECs with ratios of 25%:75%, 50%:50% and 75%:25% were performed. We were able to prove that cocultivation supports proliferation whereas apoptosis was unidirectional decreased in cocultured HUVECs mediated by a p-BAD-dependent mechanism. Moreover, coculturing ADSCs and HUVECs stimulated matrix mineralization and the activity of alkaline phosphatase (ALP). Increased gene expression of the proangiogenic markers eNOS, Flt, Ang2 and MMP3 as well as sprouting phenomena in matrigel assays proved the angiogenic potential of the coculture. In summary, coculturing ADSCs and HUVECs stimulates proliferation, cell survival, osteogenesis and angiogenesis particularly in the 50%:50% coculture.
Identifiants
pubmed: 32394620
doi: 10.1111/jcmm.15374
pmc: PMC7299704
doi:
Substances chimiques
Core Binding Factor Alpha 1 Subunit
0
Drug Combinations
0
Laminin
0
Proteoglycans
0
matrigel
119978-18-6
Collagen
9007-34-5
Alkaline Phosphatase
EC 3.1.3.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7034-7043Informations de copyright
© 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
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