Target enrichment from a DNA mixture by oligoribonucleotide interference-PCR (ORNi-PCR).
16S rRNA genes
ORNi-PCR
PCR
genome editing
microbiome profiling
oligoribonucleotide
Journal
Biology methods & protocols
ISSN: 2396-8923
Titre abrégé: Biol Methods Protoc
Pays: England
ID NLM: 101693064
Informations de publication
Date de publication:
2019
2019
Historique:
received:
27
04
2019
revised:
20
06
2019
accepted:
21
06
2019
entrez:
13
5
2020
pubmed:
13
5
2020
medline:
13
5
2020
Statut:
epublish
Résumé
Oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) is a method that suppresses PCR amplification of target DNA in an ORN-specific manner. In this study, we examined whether ORNi-PCR can be used to enrich desirable DNA sequences from a DNA mixture by suppressing undesirable DNA amplification. ORNi-PCR enriched edited DNA sequences from a mixture of genomic DNA subjected to genome editing. ORNi-PCR enabled more efficient analysis of the types of insertion/deletion mutations introduced by genome editing. In addition, ORNi-PCR reduced the detection of
Identifiants
pubmed: 32395627
doi: 10.1093/biomethods/bpz009
pii: bpz009
pmc: PMC7200947
doi:
Types de publication
Journal Article
Langues
eng
Pagination
bpz009Informations de copyright
© The Author(s) 2019. Published by Oxford University Press.
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