Proteomic analysis of "Oriental Beauty" oolong tea leaves with different degrees of leafhopper infestation.


Journal

Rapid communications in mass spectrometry : RCM
ISSN: 1097-0231
Titre abrégé: Rapid Commun Mass Spectrom
Pays: England
ID NLM: 8802365

Informations de publication

Date de publication:
15 Aug 2020
Historique:
received: 13 02 2020
revised: 03 04 2020
accepted: 30 04 2020
pubmed: 13 5 2020
medline: 20 4 2021
entrez: 13 5 2020
Statut: ppublish

Résumé

Oriental Beauty, a type of oolong tea native to Taiwan, is highly prized by connoisseurs for its unique fruity aroma and sweet taste. Leaves of Oriental Beauty vary in appearance, aroma, and taste, depending on the degree of tea green leafhopper (Jacobiasca formosana) infestation. In this study, the aim is to investigate the differential expression of proteins in leaves with low, medium, and high degrees of leafhopper infestation. Proteomic techniques 2DE (two-dimensional electrophoresis) and nanoscale liquid chromatography/tandem mass spectrometry (LC/MS/MS) were used to investigate the differential expression of proteins in tea leaves with different degrees of leafhopper infestation. A total of 89 proteins were found to exhibit significant differences in expression. In a gene ontology analysis, most of these proteins participated in biosynthesis, carbohydrate metabolism, transport, responses to stress, and amino acid metabolism. These results indicated that the unique aroma and taste of the leaves might be influenced by their protein expression profiles, as well as related factors such as defensive responses to tea green leafhopper saliva.

Identifiants

pubmed: 32396680
doi: 10.1002/rcm.8825
doi:

Substances chimiques

Flavoring Agents 0
Plant Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e8825

Informations de copyright

© 2020 John Wiley & Sons, Ltd.

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Auteurs

Han-Ju Chien (HJ)

Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan.

Man-Miao Yang (MM)

Department of Entomology, National Chung Hsing University, Taichung, Taiwan.

Wei-Chen Wang (WC)

Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan.

Xiang-Gui Hong (XG)

Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan.

Yi-Feng Zheng (YF)

Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan.

Jie-Teng Toh (JT)

Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan.

Chia-Chang Wu (CC)

Tea Research and Extension Station, Taoyuan, Taiwan.

Chien-Chen Lai (CC)

Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan.
Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan.
Advanced Plant Biotechnology Center, National Chung Hsing University, Taichung, Taiwan.

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