A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells.

Cell Differentiation Humans Muscle Development Muscle, Skeletal Pluripotent Stem Cells Transcription Factors
development human myogenesis pluripotent stem cells satellite cells single cell RNA-sequencing skeletal muscle

Journal

Cell stem cell
ISSN: 1875-9777
Titre abrégé: Cell Stem Cell
Pays: United States
ID NLM: 101311472

Informations de publication

Date de publication:
02 07 2020
Historique:
received: 17 09 2019
revised: 12 03 2020
accepted: 23 04 2020
pubmed: 13 5 2020
medline: 28 4 2021
entrez: 13 5 2020
Statut: ppublish

Résumé

The developmental trajectory of human skeletal myogenesis and the transition between progenitor and stem cell states are unclear. We used single-cell RNA sequencing to profile human skeletal muscle tissues from embryonic, fetal, and postnatal stages. In silico, we identified myogenic as well as other cell types and constructed a "roadmap" of human skeletal muscle ontogeny across development. In a similar fashion, we also profiled the heterogeneous cell cultures generated from multiple human pluripotent stem cell (hPSC) myogenic differentiation protocols and mapped hPSC-derived myogenic progenitors to an embryonic-to-fetal transition period. We found differentially enriched biological processes and discovered co-regulated gene networks and transcription factors present at distinct myogenic stages. This work serves as a resource for advancing our knowledge of human myogenesis. It also provides a tool for a better understanding of hPSC-derived myogenic progenitors for translational applications in skeletal muscle-based regenerative medicine.

Identifiants

DOI: 10.1016/j.stem.2020.04.017 PMID: 32396864 PMC: PMC7367475
pubmed: 32396864
pii: S1934-5909(20)30156-9
doi: 10.1016/j.stem.2020.04.017
pmc: PMC7367475
mid: NIHMS1590276
pii:
doi:

Substances chimiques

Transcription Factors 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

158-176.e10

Subventions

Organisme : NIMH NIH HHS
ID : R21 MH119020
Pays : United States
Organisme : NIGMS NIH HHS
ID : P01 GM099134
Pays : United States
Organisme : NIGMS NIH HHS
ID : T34 GM008563
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NIAMS NIH HHS
ID : T32 AR065972
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA016042
Pays : United States
Organisme : NIAID NIH HHS
ID : P30 AI028697
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA009056
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR064327
Pays : United States

Commentaires et corrections

Type : ErratumIn

Informations de copyright

Published by Elsevier Inc.

Déclaration de conflit d'intérêts

Declaration of Interests C.S.Y., M.S., and A.D.P. are co-founders of and have financial interests in MyoGene Bio. The Regents of the University of California have licensed intellectual property invented by C.S.Y., M.S., and A.D.P. to MyoGene Bio. M.S. and A.D.P. serve on the scientific advisory board of MyoGene Bio. C.S.Y. is currently CEO of MyoGene Bio.

Références

Nat Protoc. 2016 Oct;11(10):1833-50
pubmed: 27583644
J Cell Sci. 2018 Jul 27;131(14):
pubmed: 30054310
BMC Bioinformatics. 2012 Jun 18;13:134
pubmed: 22708584
Nat Biotechnol. 2014 Mar;32(3):279-284
pubmed: 24463574
Nat Biotechnol. 2009 Mar;27(3):275-80
pubmed: 19252484
Physiol Rev. 2010 Oct;90(4):1337-81
pubmed: 20959618
Nat Cell Biol. 2018 Jan;20(1):46-57
pubmed: 29255171
Nat Cell Biol. 2010 Feb;12(2):153-63
pubmed: 20081841
Biol Chem. 2000 Apr;381(4):331-5
pubmed: 10839462
Bioinformatics. 2015 Sep 15;31(18):2989-98
pubmed: 26002886
Physiol Rev. 2013 Jan;93(1):23-67
pubmed: 23303905
Stem Cell Reports. 2014 Mar 20;2(4):414-26
pubmed: 24749067
Nat Med. 2014 Oct;20(10):1182-6
pubmed: 25194572
Stem Cell Reports. 2015 Sep 8;5(3):419-34
pubmed: 26352798
Cell. 2010 Feb 19;140(4):554-66
pubmed: 20178747
Nat Methods. 2015 Apr;12(4):326-8
pubmed: 25730490
Results Probl Cell Differ. 2015;56:77-98
pubmed: 25344667
Cell. 2015 May 21;161(5):1202-1214
pubmed: 26000488
J Biol Chem. 1998 Dec 25;273(52):34970-5
pubmed: 9857028
Dev Dyn. 1999 Nov;216(3):274-84
pubmed: 10590479
Nat Med. 2014 Oct;20(10):1174-81
pubmed: 25194569
Curr Opin Clin Nutr Metab Care. 2015 May;18(3):215-20
pubmed: 25807347
Nat Commun. 2016 Oct 12;7:13124
pubmed: 27731315
Stem Cell Reports. 2018 Mar 13;10(3):970-983
pubmed: 29429962
Cell Stem Cell. 2016 Dec 1;19(6):800-807
pubmed: 27641304
Cell Mol Life Sci. 2014 May;71(9):1657-71
pubmed: 24232446
Development. 2019 May 28;146(10):
pubmed: 31076486
Skelet Muscle. 2015 Jul 15;5:22
pubmed: 26180627
Development. 2019 Apr 11;146(12):
pubmed: 30890574
Cold Spring Harb Symp Quant Biol. 2008;73:317-22
pubmed: 19204065
PLoS One. 2014 Feb 28;9(2):e90398
pubmed: 24587351
Stem Cell Reports. 2017 Jul 11;9(1):12-22
pubmed: 28528701
Dev Cell. 2007 Jun;12(6):931-41
pubmed: 17543865
Curr Opin Genet Dev. 2002 Oct;12(5):548-57
pubmed: 12200160
Elife. 2020 Apr 01;9:
pubmed: 32234209
Development. 2013 Apr;140(7):1528-36
pubmed: 23482488
Development. 2017 Jun 15;144(12):2104-2122
pubmed: 28634270
Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9515-20
pubmed: 24938781
Cell. 2013 Nov 7;155(4):909-921
pubmed: 24209627
Trends Cell Biol. 2016 Jun;26(6):434-444
pubmed: 26948993
Development. 2018 Oct 29;145(21):
pubmed: 30266829
J Biol Chem. 2012 Apr 6;287(15):11616-28
pubmed: 22351757
Cell Rep. 2017 Nov 14;21(7):1994-2004
pubmed: 29141228
Stem Cell Reports. 2014 Sep 9;3(3):516-29
pubmed: 25241748
Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14665-70
pubmed: 16203988
Nat Cell Biol. 2010 Feb;12(2):143-52
pubmed: 20081842
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Front Physiol. 2014 Feb 03;5:32
pubmed: 24567722
Dev Cell. 2017 Feb 27;40(4):342-353.e10
pubmed: 28245921
Genetics. 2014 Nov;198(3):1117-26
pubmed: 25217052
Cell Rep. 2014 Nov 20;9(4):1191-201
pubmed: 25456121
Cell Rep. 2020 Mar 10;30(10):3583-3595.e5
pubmed: 32160558
Nat Biotechnol. 2018 Jun;36(5):411-420
pubmed: 29608179
Mol Cell. 2019 May 2;74(3):609-621.e6
pubmed: 30922843
Science. 2014 Mar 14;343(6176):1253-6
pubmed: 24626928
Stem Cell Reports. 2016 Aug 9;7(2):263-78
pubmed: 27509136
Cell Stem Cell. 2010 Feb 5;6(2):117-29
pubmed: 20144785
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50
pubmed: 16199517
Nature. 2008 Nov 27;456(7221):502-6
pubmed: 18806774
J Cell Biol. 2010 Sep 6;190(5):867-79
pubmed: 20819939
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Cell. 2013 Sep 12;154(6):1370-9
pubmed: 23992847
Cell Rep. 2016 Mar 1;14(8):1940-52
pubmed: 26904948
Semin Cell Dev Biol. 2017 Dec;72:87-98
pubmed: 29107681
Stem Cell Reports. 2014 Jan 14;2(1):92-106
pubmed: 24678452
Nat Methods. 2017 Sep 29;14(10):935-936
pubmed: 28960196
Genome Biol. 2012 Oct 22;13(10):251
pubmed: 23088445
Cell Rep. 2017 Feb 7;18(6):1573-1585
pubmed: 28178531
Nature. 2019 Feb;566(7745):496-502
pubmed: 30787437
Mol Cells. 2019 Mar 31;42(3):200-209
pubmed: 30884942
Stem Cell Reports. 2013 Nov 27;1(6):620-31
pubmed: 24371814
FEBS J. 2013 Sep;280(17):4004-13
pubmed: 23402377
Nat Rev Mol Cell Biol. 2016 Mar;17(3):183-93
pubmed: 26883003
Science. 2016 Apr 8;352(6282):189-96
pubmed: 27124452
Nature. 2018 Oct;562(7727):367-372
pubmed: 30283141
Nat Commun. 2019 Apr 3;10(1):1523
pubmed: 30944313
Science. 2013 Feb 15;339(6121):823-6
pubmed: 23287722
Development. 1995 Mar;121(3):661-9
pubmed: 7720574
Nat Biotechnol. 2015 Sep;33(9):962-9
pubmed: 26237517
Cell Rep. 2017 Nov 14;21(7):1982-1993
pubmed: 29141227
Cell Stem Cell. 2015 Feb 5;16(2):171-83
pubmed: 25600643
Curr Opin Genet Dev. 2019 Aug;57:84-90
pubmed: 31470291
Bioinformatics. 2010 Mar 15;26(6):841-2
pubmed: 20110278
Dev Dyn. 2004 Feb;229(2):380-92
pubmed: 14745964
Sci Rep. 2020 Jan 14;10(1):229
pubmed: 31937892
Nucleic Acids Res. 2010 Jan;38(Database issue):D792-9
pubmed: 19906719
Semin Cell Dev Biol. 2007 Dec;18(6):870-82
pubmed: 18023213
Cell Rep. 2019 Jun 25;27(13):3939-3955.e6
pubmed: 31242425

Auteurs

Haibin Xi (H)

Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, USA.

Justin Langerman (J)

Deparment of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.

Shan Sabri (S)

Deparment of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.

Peggie Chien (P)

Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA.

Courtney S Young (CS)

Department of Neurology, University of California, Los Angeles, Los Angeles, CA, USA.

Shahab Younesi (S)

Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, USA.

Michael Hicks (M)

Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, USA.

Karen Gonzalez (K)

Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA.

Wakana Fujiwara (W)

Department of Biochemistry, University of California, Los Angeles, Los Angeles, CA, USA.

Julia Marzi (J)

Department of Women's Health, Research Institute for Women's Health, Eberhard Karls University Tübingen, Tübingen, Germany; The Natural and Medical Sciences Institute (NMI) at the University of Tübingen, Reutlingen, Germany.

Simone Liebscher (S)

Department of Women's Health, Research Institute for Women's Health, Eberhard Karls University Tübingen, Tübingen, Germany.

Melissa Spencer (M)

Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA; Department of Neurology, University of California, Los Angeles, Los Angeles, CA, USA.

Ben Van Handel (B)

Department of Orthopaedic Surgery, Keck School of Medicine, Stem Cell Research and Regenerative Medicine, University of Southern California, Los Angeles, CA, USA.

Denis Evseenko (D)

Department of Orthopaedic Surgery, Keck School of Medicine, Stem Cell Research and Regenerative Medicine, University of Southern California, Los Angeles, CA, USA.

Katja Schenke-Layland (K)

Department of Women's Health, Research Institute for Women's Health, Eberhard Karls University Tübingen, Tübingen, Germany; The Natural and Medical Sciences Institute (NMI) at the University of Tübingen, Reutlingen, Germany; Department of Medicine/Cardiology, Cardiovascular Research Laboratories, University of California, Los Angeles, Los Angeles, CA, USA.

Kathrin Plath (K)

Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, USA; Deparment of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA; Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, CA, USA. Electronic address: kplath@mednet.ucla.edu.

April D Pyle (AD)

Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA; Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, CA, USA. Electronic address: apyle@mednet.ucla.edu.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › A Human Skeletal Muscle Atlas Identifies the...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › A Human Skeletal Muscle Atlas Identifies the...
questionsmedicales.fr › Phénomènes physiologiques de l'appareil locomoteur et du système nerveux › Phénomènes physiologiques du système locomoteur › Développement locomoteur › Développement musculaire › A Human Skeletal Muscle Atlas Identifies the...
questionsmedicales.fr › Tissus › Muscles › Muscle strié › Muscles squelettiques › A Human Skeletal Muscle Atlas Identifies the...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › A Human Skeletal Muscle Atlas Identifies the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › A Human Skeletal Muscle Atlas Identifies the...