Equine penile squamous cell carcinoma: expression of biomarker proteins and EcPV2.
Animals
Biomarkers, Tumor
/ genetics
Carcinoma, Squamous Cell
/ diagnosis
Cyclin D1
/ genetics
Gene Expression Regulation, Neoplastic
Horses
Male
Matrix Metalloproteinase 7
/ genetics
Papillomaviridae
/ isolation & purification
Papillomavirus Infections
/ diagnosis
Penile Neoplasms
/ diagnosis
Protein Binding
Proto-Oncogene Proteins c-fos
/ genetics
Proto-Oncogene Proteins c-myc
/ genetics
ROC Curve
Tissue Array Analysis
/ methods
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
12 05 2020
12 05 2020
Historique:
received:
06
05
2019
accepted:
27
03
2020
entrez:
14
5
2020
pubmed:
14
5
2020
medline:
1
12
2020
Statut:
epublish
Résumé
Equine penile squamous cell carcinoma (EpSCC) is a relatively common cutaneous neoplasm with a poor prognosis. In this study, we aimed to determine the protein expression and colocalisation of FRA1, c-Myc, Cyclin D1, and MMP7 in normal (NT), tumour (T), hyperplastic epidermis and/or squamous papilloma (Hyp/Pap), poorly-differentiated (PDSCC), or well-differentiated (WDSCC) EpSCC using a tissue array approach. Further objectives were to correlate protein expression to (i) levels of inflammation, using a convolutional neural network (ii) equine papillomavirus 2 (EcPV2) infection, detected using PCR amplification. We found an increase in expression of FRA1 in EpSCC compared to NT samples. c-Myc expression was higher in Hyp/Pap and WDSCC but not PDSCC whereas MMP7 was reduced in WDSCC compared with NT. There was a significant increase in the global intersection coefficient (GIC) of FRA1 with MMP7, c-Myc, and Cyclin D1 in EpSCC. Conversely, GIC for MMP7 with c-Myc was reduced in EpSCC tissue. Inflammation was positively associated with EcPV2 infection in both NT and EpSCC but not Hyp/Pap. Changes in protein expression could be correlated with EcPV2 for Cyclin D1 and c-Myc. Our results evaluate novel biomarkers of EpSCC and a putative correlation between the expression of biomarkers, EcPV2 infection and inflammation.
Identifiants
pubmed: 32398763
doi: 10.1038/s41598-020-64014-3
pii: 10.1038/s41598-020-64014-3
pmc: PMC7217868
doi:
Substances chimiques
Biomarkers, Tumor
0
Proto-Oncogene Proteins c-fos
0
Proto-Oncogene Proteins c-myc
0
fos-related antigen 1
0
Cyclin D1
136601-57-5
Matrix Metalloproteinase 7
EC 3.4.24.23
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7863Subventions
Organisme : Medical Research Council
ID : MR/N022556/1
Pays : United Kingdom
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