Isolation of Secreted and Tegumental Surface Proteins from Fasciola hepatica.
Excretory–secretory products
Fasciola hepatica
Host–parasite interaction
Proteomics
Secretome
Tegument
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
14
5
2020
pubmed:
14
5
2020
medline:
12
3
2021
Statut:
ppublish
Résumé
Proteins secreted by, or displayed on the surface tegument of, trematodes have key functions in the host-parasite interaction. As such, they are often leading targets for diagnostic tests or vaccine candidates. Here we describe methods for the isolation and analysis of soluble secreted proteins (i.e., the secretome) released during in vitro culture of adult Fasciola hepatica. We also describe two methods for the enrichment of proteins displayed on the outer tegumental surface of F. hepatica. These approaches enable downstream identification of the isolated proteins by mass spectrometry-based proteomics.
Identifiants
pubmed: 32399919
doi: 10.1007/978-1-0716-0475-5_3
doi:
Substances chimiques
Helminth Proteins
0
Membrane Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
27-36Références
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