Activation of Transient Receptor Potential Melastatin Family Member 8 (TRPM8) Receptors Induces Proinflammatory Cytokine Expressions in Bronchial Epithelial Cells.
Asthma
epithelium
interleukin-25
thymic stromal lymphopoietin
transient receptor potential channels
Journal
Allergy, asthma & immunology research
ISSN: 2092-7355
Titre abrégé: Allergy Asthma Immunol Res
Pays: Korea (South)
ID NLM: 101518382
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
11
12
2019
revised:
25
02
2020
accepted:
02
03
2020
entrez:
14
5
2020
pubmed:
14
5
2020
medline:
14
5
2020
Statut:
ppublish
Résumé
Cold air is a major environmental factor that exacerbates asthma. Transient receptor potential melastatin family member 8 (TRPM8) is a cold-sensing channel expressed in the airway epithelium. However, its role in airway inflammation remains unknown. We investigated the role of TRPM8 in innate immune responses in bronchial epithelial cells and asthmatic subjects. The TRPM8 mRNA and protein expression on BEAS2B human bronchial epithelial cells was examined by real-time polymerase chain reaction (PCR), immunofluorescence staining and western blotting. Additionally, interleukin (IL)-4, IL-6, IL-8, IL-13, IL-25 and thymic stromal lymphopoietin (TSLP) levels before and after menthol, dexamethasone and N-(4-tert-butylphenyl)-4-(3-chloropyridin-2-yl) piperazine-1-carboxamide (BCTC) treatments were measured via real-time PCR. TRPM8 protein levels in the supernatants of induced sputum from asthmatic subjects and normal control subjects were measured using enzyme-linked immunosorbent assay, and mRNA levels in sputum cell lysates were measured using real-time PCR. Treatment with up to 2 mM menthol dose-dependently increased TRPM8 mRNA and protein in BEAS2B cells compared to untreated cells ( TRPM8 may be involved in the airway epithelial cell innate immune response and a molecular target for the treatment of asthma.
Identifiants
pubmed: 32400133
pii: 12.684
doi: 10.4168/aair.2020.12.4.684
pmc: PMC7224991
doi:
Types de publication
Journal Article
Langues
eng
Pagination
684-700Subventions
Organisme : National Research Foundation of Korea
ID : NFR-2017R1C1B5076565
Pays : Korea
Organisme : Hallym University Medical Center
ID : 01-2012-12
Pays : Korea
Informations de copyright
Copyright © 2020 The Korean Academy of Asthma, Allergy and Clinical Immunology · The Korean Academy of Pediatric Allergy and Respiratory Disease.
Déclaration de conflit d'intérêts
There are no financial or other issues that might lead to conflict of interest.
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