Effects of oxidative stress on liver, brain and spinal cord of rats using L-NAME and treated with hydroxyurea. A model of sickle cell complication.
Journal
Acta cirurgica brasileira
ISSN: 1678-2674
Titre abrégé: Acta Cir Bras
Pays: Brazil
ID NLM: 9103983
Informations de publication
Date de publication:
2020
2020
Historique:
received:
12
11
2019
accepted:
10
02
2020
entrez:
14
5
2020
pubmed:
14
5
2020
medline:
27
5
2020
Statut:
ppublish
Résumé
To analyze the serum levels of nitric oxide and correlate them with the levels of thiobarbituric acid reactive substances (TBARS) in liver, brain and spinal cord of animals using L-NAME and treated with hydroxyurea. Eighteen male albino Wistar rats were divided into three groups. NG-nitro-L-arginine methyl ester (L-NAME) was intraperitoneally administered to induce oxidative stress. TBARS and plasma nitric oxide levels were analyzed in all groups. Histopathology of the liver and vascular tissue was performed. Statistically significant differences were seen in liver, brain and spinal cord TBARS levels. Following the use of L-NAME, hepatic tissue increased the number of Kupffer cells as oxidative stress and inflammatory response increased. The use of L-NAME caused an increase in lipid peroxidation products and, consequently, in oxidative stress in animals. Hydroxyurea doses of 35 mg / kg / day reduced TBARS values in liver, brain and spinal cord.
Identifiants
pubmed: 32401830
pii: S0102-86502020000300200
doi: 10.1590/s0102-865020200030000001
pmc: PMC7217595
pii:
doi:
Substances chimiques
NG-Nitroarginine Methyl Ester
V55S2QJN2X
Hydroxyurea
X6Q56QN5QC
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202000301Références
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