Validation of an antimicrobial susceptibility testing protocol for Brachyspira hyodysenteriae and Brachyspira pilosicoli in an international ring trial.
Antimicrobial susceptibility testing
Brachyspira
Ring trial
lnu(C)
tva(A)
tva(B)
Journal
Veterinary microbiology
ISSN: 1873-2542
Titre abrégé: Vet Microbiol
Pays: Netherlands
ID NLM: 7705469
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
05
02
2020
revised:
27
02
2020
accepted:
17
03
2020
entrez:
14
5
2020
pubmed:
14
5
2020
medline:
31
12
2020
Statut:
ppublish
Résumé
Brachyspira hyodysenteriae and Brachyspira pilosicoli cause economically important enteric disease in pigs. Treatment of these infections often includes antimicrobial administration, which can be most effective when therapeutic options are informed by antimicrobial susceptibility testing data. Here we describe a method for broth dilution antimicrobial susceptibility testing of these bacteria, both of which are difficult to culture in vitro. The protocol was evaluated for its fitness for use in an inter-laboratory ring trial involving eight laboratories from seven countries, and employing eleven test strains (5 Brachyspira hyodysenteriae including the type strain B78
Identifiants
pubmed: 32402332
pii: S0378-1135(20)30143-7
doi: 10.1016/j.vetmic.2020.108645
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Types de publication
Journal Article
Multicenter Study
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
108645Informations de copyright
Crown Copyright © 2020. Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest None; except MP and AL who are employed at the National Veterinary Institute (SVA), the former producer of VetMIC Brachy. MP and AL did not take part in the data evaluation and analyses.