Leukoreduction in ex vivo perfusion circuits: comparison of leukocyte depletion efficiency with leukocyte filters.
blood based perfusate
ex vivo perfusion
leukocyte filter
leukodepletion
leukoreduction
perfusate
Journal
Perfusion
ISSN: 1477-111X
Titre abrégé: Perfusion
Pays: England
ID NLM: 8700166
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
pubmed:
15
5
2020
medline:
12
8
2021
entrez:
15
5
2020
Statut:
ppublish
Résumé
Leukodepletion of whole blood-based perfusates remains a challenge in experimental models of ex vivo perfusion. This study investigated the leukoreduction efficacy of the commonly used LeukoGuard LG Arterial and BC2 Cardioplegia filters. Eleven liters of washed porcine blood was used to evaluate the filtration efficiency of LG (n = 6) and BC2 (n = 5) filters. Filter efficacy was tested by passing 1 L of washed blood through each filter. Complete blood count was performed to detect a reduction of white blood cells, red blood cells, and hemoglobin concentration. The BC2 Cardioplegia filter showed a significant reduction in white blood cell count (13.16 ± 4.2 × 10 Our results suggest that the LG filter should not be used in ex vivo perfusion circuits for the purpose of leukodepletion. The BC2 filter can be used in EVP circuits with flow rates of less than 350 mL/min. Alternatively, perfusate may be leukodepleted before perfusion.
Sections du résumé
BACKGROUND
Leukodepletion of whole blood-based perfusates remains a challenge in experimental models of ex vivo perfusion. This study investigated the leukoreduction efficacy of the commonly used LeukoGuard LG Arterial and BC2 Cardioplegia filters.
METHODS
Eleven liters of washed porcine blood was used to evaluate the filtration efficiency of LG (n = 6) and BC2 (n = 5) filters. Filter efficacy was tested by passing 1 L of washed blood through each filter. Complete blood count was performed to detect a reduction of white blood cells, red blood cells, and hemoglobin concentration.
RESULTS
The BC2 Cardioplegia filter showed a significant reduction in white blood cell count (13.16 ± 4.2 × 10
CONCLUSION
Our results suggest that the LG filter should not be used in ex vivo perfusion circuits for the purpose of leukodepletion. The BC2 filter can be used in EVP circuits with flow rates of less than 350 mL/min. Alternatively, perfusate may be leukodepleted before perfusion.
Identifiants
pubmed: 32404024
doi: 10.1177/0267659120917872
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM