The Protective Effect of Zinc Against Liver Ischaemia Reperfusion Injury in a Rat Model of Global Ischaemia.
ALT, Alanine Transaminase
ANOVA, Analysis of Variance
AST, Aspartate Transaminase
IRI, Ischaemia-Reperfusion Injury
injury
ischaemia
liver
reperfusion
zinc
Journal
Journal of clinical and experimental hepatology
ISSN: 0973-6883
Titre abrégé: J Clin Exp Hepatol
Pays: India
ID NLM: 101574137
Informations de publication
Date de publication:
Historique:
received:
04
04
2019
accepted:
16
07
2019
entrez:
15
5
2020
pubmed:
15
5
2020
medline:
15
5
2020
Statut:
ppublish
Résumé
Ischaemia-reperfusion injury (IRI) is a major obstacle during liver transplantation and resection surgeries for cancer, with a need for effective and safe drugs to reduce IRI. Zinc preconditioning has been shown to protect against liver IRI in a partial (70%) ischaemia model. However, its efficacy against a clinically relevant Pringle manoeuvre that results in global liver ischaemia (100%) is unknown. The aim of this study was to test the efficacy of zinc preconditioning in a rat model of global liver ischaemia. Rats were preconditioned via subcutaneous injection of 10 mg/kg of ZnCl On a two-way repeated-measures analysis of variance, there was a statistically significant (p = 0.025) difference in the mean ALT levels between saline- and ZnCl Zinc preconditioning reduces the overall hepatocellular damage from IRI. These results lay the foundation to assess the benefit of zinc preconditioning for clinical applications.
Sections du résumé
BACKGROUND
BACKGROUND
Ischaemia-reperfusion injury (IRI) is a major obstacle during liver transplantation and resection surgeries for cancer, with a need for effective and safe drugs to reduce IRI. Zinc preconditioning has been shown to protect against liver IRI in a partial (70%) ischaemia model. However, its efficacy against a clinically relevant Pringle manoeuvre that results in global liver ischaemia (100%) is unknown.
AIMS
OBJECTIVE
The aim of this study was to test the efficacy of zinc preconditioning in a rat model of global liver ischaemia.
METHODS
METHODS
Rats were preconditioned via subcutaneous injection of 10 mg/kg of ZnCl
RESULTS
RESULTS
On a two-way repeated-measures analysis of variance, there was a statistically significant (p = 0.025) difference in the mean ALT levels between saline- and ZnCl
CONCLUSION
CONCLUSIONS
Zinc preconditioning reduces the overall hepatocellular damage from IRI. These results lay the foundation to assess the benefit of zinc preconditioning for clinical applications.
Identifiants
pubmed: 32405179
doi: 10.1016/j.jceh.2019.07.006
pii: S0973-6883(19)30200-2
pmc: PMC7212296
doi:
Types de publication
Journal Article
Langues
eng
Pagination
228-235Informations de copyright
© 2019 Indian National Association for Study of the Liver. Published by Elsevier B.V. All rights reserved.
Références
Hepatology. 2008 Feb;47(2):461-72
pubmed: 18098300
J Clin Chem Clin Biochem. 1986 Jul;24(7):497-510
pubmed: 3734712
Oncotarget. 2018 Jan 3;9(9):8463-8477
pubmed: 29492208
Adv Shock Res. 1982;8:177-86
pubmed: 7136943
Biochim Biophys Acta Mol Basis Dis. 2017 Feb;1863(2):375-385
pubmed: 27989959
Acta Chir Scand. 1982;148(7):601-7
pubmed: 7168290
Acta Cir Bras. 2007 Jul-Aug;22(4):279-84
pubmed: 17625666
Tohoku J Exp Med. 1982 Sep;138(1):63-70
pubmed: 6293117
Lab Anim (NY). 2009 Mar;38(3):87-93
pubmed: 19229225
Int J Mol Sci. 2018 Apr 26;19(5):
pubmed: 29701719
Liver Transpl. 2015 Apr;21(4):487-99
pubmed: 25545865
Int Surg. 2002 Jan-Mar;87(1):25-30
pubmed: 12144186
Toxicol In Vitro. 2009 Jun;23(4):653-9
pubmed: 19306923
Eur J Cardiothorac Surg. 2012 Jan;41(1):193-9
pubmed: 21665487
Gastroenterology. 2003 Sep;125(3):917-36
pubmed: 12949736
HPB (Oxford). 2010 Sep;12(7):439-46
pubmed: 20815852
J Pharmacol Exp Ther. 2007 May;321(2):517-25
pubmed: 17322024
Clin Biochem. 1990 Aug;23(4):311-9
pubmed: 2225456
Nat Med. 2011 Nov 07;17(11):1391-401
pubmed: 22064429
Metallomics. 2015 Jan;7(1):102-11
pubmed: 25360687
Acta Cir Bras. 2018 Aug;33(8):723-735
pubmed: 30208134
Exp Biol Med (Maywood). 2010 Jun;235(6):741-50
pubmed: 20511678
Biomed Pharmacother. 2017 Dec;96:48-55
pubmed: 28963950
J Basic Clin Pharm. 2016 Mar;7(2):27-31
pubmed: 27057123
Arch Surg. 1999 May;134(5):533-9
pubmed: 10323426
PLoS One. 2017 Jul 7;12(7):e0180028
pubmed: 28686686
Metallomics. 2015 Oct;7(10):1390-8
pubmed: 26404630
J Biomed Biotechnol. 2012;2012:298657
pubmed: 22649277
Transplantation. 1993 Jun;55(6):1265-72
pubmed: 7685932
J Gastroenterol Hepatol. 2011 Nov;26(11):1652-8
pubmed: 22011298
Biometals. 2018 Oct;31(5):821-834
pubmed: 29974287
HPB (Oxford). 2009 May;11(3):264-73
pubmed: 19590658
Sci Rep. 2015 Sep 24;5:14406
pubmed: 26400669
Ann Surg Innov Res. 2010 Mar 26;4:2
pubmed: 20346153
Arq Bras Cir Dig. 2014 Jul-Sep;27(3):196-200
pubmed: 25184771
CMAJ. 2005 Feb 1;172(3):367-79
pubmed: 15684121