Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Is Comparable in Clinical Samples Preserved in Saline or Viral Transport Medium.
Betacoronavirus
/ isolation & purification
COVID-19
COVID-19 Testing
Clinical Laboratory Techniques
/ methods
Coronavirus Infections
/ diagnosis
Humans
Pandemics
/ prevention & control
Pneumonia, Viral
/ diagnosis
Predictive Value of Tests
Preservation, Biological
RNA, Viral
/ analysis
SARS-CoV-2
Saline Solution
/ chemistry
Specimen Handling
/ methods
Virus Cultivation
/ methods
Journal
The Journal of molecular diagnostics : JMD
ISSN: 1943-7811
Titre abrégé: J Mol Diagn
Pays: United States
ID NLM: 100893612
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
18
04
2020
accepted:
28
04
2020
pubmed:
15
5
2020
medline:
14
7
2020
entrez:
15
5
2020
Statut:
ppublish
Résumé
As the coronavirus disease 2019 (COVID-19) pandemic sweeps across the world, the availability of viral transport medium (VTM) has become severely limited, contributing to delays in diagnosis and rationing of diagnostic testing. Given that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA has demonstrated stability, we posited that phosphate-buffered saline (PBS) may be a viable transport medium, as an alternative to VTM, for clinical real-time quantitative PCR (qPCR) testing. The intra-individual reliability and interindividual reliability of SARS-CoV-2 qPCR were assessed in clinical endotracheal secretion samples transported in VTM or PBS to evaluate the stability of the qPCR signal for three viral targets (N gene, ORF1ab, and S gene) when samples were stored in these media at room temperature for up to 18 hours. We report that the use of PBS as a transport medium allows high intra-individual and interindividual reliability, maintains viral stability, and compares with VTM in the detection of the three SARS-CoV-2 genes through 18 hours of storage. This study establishes PBS as a clinically useful medium that can be readily deployed for transporting and short-term preservation of specimens containing SARS-CoV-2. Use of PBS as a transport medium has the potential to increase testing capacity for SARS-CoV-2, aiding more widespread screening and early diagnosis of COVID-19.
Identifiants
pubmed: 32405270
doi: 10.1016/j.jmoldx.2020.04.209
pii: S1525-1578(20)30323-8
pmc: PMC7219422
pii:
doi:
Substances chimiques
RNA, Viral
0
Saline Solution
0
Types de publication
Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
871-875Subventions
Organisme : NHLBI NIH HHS
ID : P01 HL114471
Pays : United States
Organisme : NHLBI NIH HHS
ID : U01 HL133817
Pays : United States
Organisme : NIEHS NIH HHS
ID : P30 ES005022
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR003017
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL149450
Pays : United States
Organisme : NIAID NIH HHS
ID : UH3 AI122309
Pays : United States
Organisme : NIAMS NIH HHS
ID : K23 AR070286
Pays : United States
Informations de copyright
Copyright © 2020 Association for Molecular Pathology and American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.
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