Dietary lipids accumulate in macrophages and stromal cells and change the microarchitecture of mesenteric lymph nodes.
Lipid droplets
Lymphatic system
Microarray
Obesity
Journal
Journal of advanced research
ISSN: 2090-1232
Titre abrégé: J Adv Res
Pays: Egypt
ID NLM: 101546952
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
11
11
2019
revised:
24
04
2020
accepted:
28
04
2020
entrez:
15
5
2020
pubmed:
15
5
2020
medline:
15
5
2020
Statut:
epublish
Résumé
In obesity, increased dietary lipids are taken up and transported by the lymphatic systems into the circulatory system. Increased fat accumulation results in impairments in the lymph fluid and lymph node (LN) atrophy. LNs filter the lymph fluid for foreign antigens to induce and control immune responses, and the alteration of this function during obesity remains underexplored. Here, the changes within the microarchitecture of mesenteric LNs (mLNs) during high levels of lipid transport were investigated, and the role of stromal cells in mice fed a high-fat diet for 10 weeks was assessed. Microarray experiments revealed that gene probes involved in lipid metabolism are expressed by mLN stromal cells. Transmission electron microscopy enabled the identification of lipid droplets in lymphatic endothelial cells, different reticulum cells, and macrophages, and the lipid droplet sizes as well as their numbers and intercellular distances increased after 10 weeks of high-fat diet feeding. The results indicate that changes in the microarchitecture and increased accumulation of lipid droplets in stromal cells and macrophages influence the immunological function of mLNs.
Identifiants
pubmed: 32405435
doi: 10.1016/j.jare.2020.04.020
pii: S2090-1232(20)30082-5
pmc: PMC7210474
doi:
Types de publication
Journal Article
Langues
eng
Pagination
291-300Informations de copyright
© 2020 THE AUTHORS. Published by Elsevier BV on behalf of Cairo University.
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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