Comparison of 2 Serum-Free Light-Chain Assays in CKD Patients.
assay
chronic kidney disease
free light chain
Journal
Kidney international reports
ISSN: 2468-0249
Titre abrégé: Kidney Int Rep
Pays: United States
ID NLM: 101684752
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
26
08
2019
revised:
10
01
2020
accepted:
23
01
2020
entrez:
15
5
2020
pubmed:
15
5
2020
medline:
15
5
2020
Statut:
epublish
Résumé
Quantification of serum-free light chains (FLCs) is important in the diagnosis and monitoring of paraprotein-related diseases. There are currently 2 FLC assays available: the Freelite assay (Binding Site) and the N Latex assay (Siemens). There is emerging evidence that these assays give different results, but it is not established how kidney dysfunction affects these assays differently. In this study, we measured and compared serum FLCs in patients with mild-to-moderate chronic kidney disease (CKD) using both assays. Although κ FLCs are higher by Freelite, λ FLCs are higher by N Latex. Both κ and λ FLCs correlate inversely with estimated glomerular filtration rate (eGFR) in the 2 assays, but this effect is more pronounced in λ-free light-chain measurement by N Latex. Consequently, although the κ/λ ratio by Freelite is inversely correlated by eGFR, the κ/λ ratio by N Latex is positively correlated with eGFR. Our results clearly demonstrate that the 2 available FLC assays cannot be used interchangeably in patients with CKD.
Identifiants
pubmed: 32405584
doi: 10.1016/j.ekir.2020.01.019
pii: S2468-0249(20)30040-1
pmc: PMC7210599
doi:
Types de publication
Journal Article
Langues
eng
Pagination
627-631Informations de copyright
© 2020 International Society of Nephrology. Published by Elsevier Inc.
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