Correlative imaging of collagen fibers and fibroblasts using CLEM optimized for picrosirius red staining and FIB/SEM tomography.
CLEM
FIB/SEM
collagen
fibroblast
picrosirius red staining
three-dimensional imaging
Journal
Microscopy (Oxford, England)
ISSN: 2050-5701
Titre abrégé: Microscopy (Oxf)
Pays: England
ID NLM: 101595834
Informations de publication
Date de publication:
30 Oct 2020
30 Oct 2020
Historique:
received:
09
03
2020
revised:
16
04
2020
accepted:
07
05
2020
pubmed:
15
5
2020
medline:
5
6
2021
entrez:
15
5
2020
Statut:
ppublish
Résumé
Conventional imaging for three-dimensional (3D) ultra-architectural analysis of collagen fibers and fibroblasts is time-consuming and requires numerous ultrathin sections to search the target area. Currently, no method allows 3D ultra-architectural analysis of predetermined areas including spatial relationships between collagen fibers and fibroblasts in vitro. Herein, we developed a new method for in vitro analysis of the 3D ultrastructure of fibroblasts and collagen fibers using CLEM optimized for picrosirius red staining and FIB/SEM tomography. Collagen fibers were observed between, rather than on top of, stacked cells. This method offers the advantage of mesoscopic and ultrastructural analysis, thus minimizing bias and ensuring accurate observation.
Identifiants
pubmed: 32406508
pii: 5836962
doi: 10.1093/jmicro/dfaa024
doi:
Substances chimiques
Azo Compounds
0
C.I. direct red 80
1294D5G72N
Collagen
9007-34-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
324-329Informations de copyright
© The Author(s) 2020. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.