Ablation of non-coding RNAs affects bovine leukemia virus B lymphocyte proliferation and abrogates oncogenesis.
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
27
01
2020
accepted:
26
03
2020
revised:
27
05
2020
pubmed:
15
5
2020
medline:
22
7
2020
entrez:
15
5
2020
Statut:
epublish
Résumé
Viruses have developed different strategies to escape from immune response. Among these, viral non-coding RNAs are invisible to the immune system and may affect the fate of the host cell. Bovine leukemia virus (BLV) encodes both short (miRNAs) and long (antisense AS1 and AS2) non-coding RNAs. To elucidate the mechanisms associated with BLV non-coding RNAs, we performed phenotypic and transcriptomic analyzes in a reverse genetics system. RNA sequencing of B-lymphocytes revealed that cell proliferation is the most significant mechanism associated with ablation of the viral non-coding RNAs. To assess the biological relevance of this observation, we determined the cell kinetic parameters in vivo using intravenous injection of BrdU and CFSE. Fitting the data to a mathematical model provided the rates of cell proliferation and death. Our data show that deletion of miRNAs correlates with reduced proliferation of the infected cell and lack of pathogenesis.
Identifiants
pubmed: 32407379
doi: 10.1371/journal.ppat.1008502
pii: PPATHOGENS-D-20-00159
pmc: PMC7252678
doi:
Substances chimiques
MicroRNAs
0
RNA, Antisense
0
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008502Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Retrovirology. 2016 May 03;13(1):33
pubmed: 27141823
Front Vet Sci. 2018 Jan 15;4:245
pubmed: 29379791
Cell Host Microbe. 2011 Nov 17;10(5):515-26
pubmed: 22100165
Genome Res. 2003 Nov;13(11):2498-504
pubmed: 14597658
J Virol. 2003 Dec;77(24):13073-83
pubmed: 14645564
Retrovirology. 2007 Mar 16;4:18
pubmed: 17362524
Virol J. 2017 Nov 2;14(1):209
pubmed: 29096657
mBio. 2014 Apr 08;5(2):e00074
pubmed: 24713319
Proc Natl Acad Sci U S A. 2006 Jan 17;103(3):720-5
pubmed: 16407133
Histopathology. 2006 May;48(6):674-82
pubmed: 16681683
Proc Natl Acad Sci U S A. 2006 May 2;103(18):7024-9
pubmed: 16641092
J Virol. 2007 Dec;81(23):12836-45
pubmed: 17881434
PLoS Pathog. 2012 Jan;8(1):e1002484
pubmed: 22291592
Science. 2012 Apr 13;336(6078):220-5
pubmed: 22422861
J Virol. 2007 Sep;81(18):10195-200
pubmed: 17626096
RNA Biol. 2018;15(12):1440-1447
pubmed: 30513054
Proc Biol Sci. 2006 May 7;273(1590):1165-71
pubmed: 16600897
Retrovirology. 2019 May 16;16(1):14
pubmed: 31096993
Annu Rev Anim Biosci. 2014 Feb;2:189-208
pubmed: 25384140
Nature. 2010 Oct 7;467(7316):719-23
pubmed: 20739936
Nature. 2009 Oct 8;461(7265):819-22
pubmed: 19783980
Genome Biol. 2014;15(12):550
pubmed: 25516281
Retrovirology. 2004 Oct 05;1:31
pubmed: 15462678
Front Microbiol. 2018 Jan 15;8:2684
pubmed: 29379479
J Virol. 2006 Feb;80(4):1922-38
pubmed: 16439548
Indian J Ophthalmol. 2011 Mar-Apr;59(2):85-6
pubmed: 21350275
J Virol. 1996 Apr;70(4):2170-7
pubmed: 8642639
Nucleic Acids Res. 2013 May 1;41(10):e108
pubmed: 23558742
Proc Natl Acad Sci U S A. 2013 Feb 5;110(6):2306-11
pubmed: 23345446
PLoS Pathog. 2013;9(10):e1003687
pubmed: 24098130
Microb Pathog. 1992 May;12(5):319-31
pubmed: 1323740
Proc Natl Acad Sci U S A. 2005 Jul 19;102(29):10309-14
pubmed: 16006517
J Virol. 2001 Aug;75(15):6977-88
pubmed: 11435578
PLoS Pathog. 2011 Feb;7(2):e1001305
pubmed: 21383974
Viruses. 2015 Nov 24;7(11):6080-8
pubmed: 26610551
Genome Res. 2009 Sep;19(9):1639-45
pubmed: 19541911
J Clin Invest. 2010 Apr;120(4):939-49
pubmed: 20364091
Nat Genet. 2003 Jul;34(3):267-73
pubmed: 12808457
Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):10048-53
pubmed: 12119390
Nat Cell Biol. 2007 Jun;9(6):654-9
pubmed: 17486113
Proc Natl Acad Sci U S A. 2010 Apr 6;107(14):6328-33
pubmed: 20304794
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50
pubmed: 16199517
Viruses. 2014 Jun 20;6(6):2416-27
pubmed: 24956179
PLoS Pathog. 2012 Dec;8(12):e1003018
pubmed: 23308061
Sci Rep. 2016 Aug 22;6:31125
pubmed: 27545598
Vet Immunol Immunopathol. 1989 Nov 30;23(1-2):139-47
pubmed: 2559534
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Curr Opin Virol. 2017 Oct;26:15-19
pubmed: 28753440
Nucleic Acids Res. 2017 Sep 29;45(17):e154
pubmed: 28973449
Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):3077-82
pubmed: 22308400
Front Biosci. 2007 Jan 01;12:1520-31
pubmed: 17127399
J Biol Chem. 2010 Nov 12;285(46):36129-37
pubmed: 20826784
J Virol. 2006 Dec;80(24):11998-2008
pubmed: 17035334
Nucleic Acids Res. 2014 Dec 16;42(22):13949-62
pubmed: 25428356
J Virol. 2012 Jan;86(1):621-4
pubmed: 22031946
J Am Vet Med Assoc. 2014 Apr 15;244(8):914-22
pubmed: 24697767
Vet Immunol Immunopathol. 2008 Sep 15;125(1-2):1-7
pubmed: 18513803
PLoS Pathog. 2016 Apr 28;12(4):e1005588
pubmed: 27123579