TENSCell: Imaging of Stretch-Activated Cells Reveals Divergent Nuclear Behavior and Tension.
Journal
Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626
Informations de publication
Date de publication:
02 06 2020
02 06 2020
Historique:
received:
18
11
2019
revised:
08
01
2020
accepted:
13
03
2020
pubmed:
15
5
2020
medline:
15
5
2021
entrez:
15
5
2020
Statut:
ppublish
Résumé
Mechanisms of cellular and nuclear mechanosensation are unclear, partially because of a lack of methods that can reveal dynamic processes. Here, we present a new concept for a low-cost, three-dimensionally printed device that enables high-magnification imaging of cells during stretch. We observed that nuclei of mouse embryonic skin fibroblasts underwent rapid (within minutes) and divergent responses, characterized by nuclear area expansion during 5% strain but nuclear area shrinkage during 20% strain. Only responses to low strain were dependent on calcium signaling, whereas actin inhibition abrogated all nuclear responses and increased nuclear strain transfer and DNA damage. Imaging of actin dynamics during stretch revealed similar divergent trends, with F-actin shifting away from (5% strain) or toward (20% strain) the nuclear periphery. Our findings emphasize the importance of simultaneous stimulation and data acquisition to capture mechanosensitive responses and suggest that mechanical confinement of nuclei through actin may be a protective mechanism during high mechanical stretch or loading.
Identifiants
pubmed: 32407683
pii: S0006-3495(20)30340-4
doi: 10.1016/j.bpj.2020.03.035
pmc: PMC7264846
pii:
doi:
Substances chimiques
Actins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
2627-2640Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR063712
Pays : United States
Organisme : NIAMS NIH HHS
ID : R21 AR066230
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM065103
Pays : United States
Informations de copyright
Copyright © 2020 Biophysical Society. Published by Elsevier Inc. All rights reserved.
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