Fluorescence labeling of a Na
Hs1a-FL
Intraoperative
Near-infrared imaging
Nerve imaging
Journal
EJNMMI research
ISSN: 2191-219X
Titre abrégé: EJNMMI Res
Pays: Germany
ID NLM: 101560946
Informations de publication
Date de publication:
14 May 2020
14 May 2020
Historique:
received:
20
02
2020
accepted:
08
04
2020
entrez:
16
5
2020
pubmed:
16
5
2020
medline:
16
5
2020
Statut:
epublish
Résumé
Accidental peripheral nerve injury during surgical intervention results in a broad spectrum of potentially debilitating side effects. Tissue distortion and poor visibility can significantly increase the risk of nerve injury with long-lasting consequences for the patient. We developed and characterized Hs1a-FL, a fluorescent near-infrared molecule for nerve visualization in the operating theater with the aim of helping physicians to visualize nerves during surgery. Hs1a was derived from the venom of the Chinese bird spider, Haplopelma schmidti, and conjugated to Cy7.5 dye. Hs1a-FL was injected intravenously in mice, and harvested nerves were imaged microscopically and with epifluorescence. Hs1a-FL showed specific and stable binding to the sodium channel Na Fluorescent Na
Sections du résumé
BACKGROUND
BACKGROUND
Accidental peripheral nerve injury during surgical intervention results in a broad spectrum of potentially debilitating side effects. Tissue distortion and poor visibility can significantly increase the risk of nerve injury with long-lasting consequences for the patient. We developed and characterized Hs1a-FL, a fluorescent near-infrared molecule for nerve visualization in the operating theater with the aim of helping physicians to visualize nerves during surgery. Hs1a was derived from the venom of the Chinese bird spider, Haplopelma schmidti, and conjugated to Cy7.5 dye. Hs1a-FL was injected intravenously in mice, and harvested nerves were imaged microscopically and with epifluorescence.
RESULTS
RESULTS
Hs1a-FL showed specific and stable binding to the sodium channel Na
CONCLUSIONS
CONCLUSIONS
Fluorescent Na
Identifiants
pubmed: 32409881
doi: 10.1186/s13550-020-00630-4
pii: 10.1186/s13550-020-00630-4
pmc: PMC7225226
doi:
Types de publication
Journal Article
Langues
eng
Pagination
49Subventions
Organisme : NIH HHS
ID : CA204441
Pays : United States
Organisme : NIH HHS
ID : CA008748
Pays : United States
Organisme : australian national health and medical research council
ID : APP1072113
Organisme : Ruth L. Kirschstein fellowship
ID : EB025050
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : Australian National Health and Medical Research Council
ID : APP1136889
Organisme : NIH HHS
ID : CA232130
Pays : United States
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