Assessing Tissue-Specific Autophagy Flux in Adult Caenorhabditis elegans.

Aging / genetics Animals Autophagy / genetics Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Longevity / genetics Microtubule-Associated Proteins / genetics Molecular Biology / methods
Aging Autophagic flux Bafilomycin A C. elegans

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 16 5 2020
pubmed: 16 5 2020
medline: 13 3 2021
Statut: ppublish

Résumé

The cellular recycling process of autophagy is essential for survival, development, and homeostasis. Autophagy also plays an important role in aging and has been linked to longevity in many species, including the nematode C. elegans. Study of the physiological roles of autophagy during C. elegans aging requires methods for the spatiotemporal analysis of autophagy. Here we describe a method for assessing autophagic flux in multiple tissues of C. elegans by quantifying the pool of autophagic vesicles using fluorescently labelled Atg8/LGG-1 reporters upon autophagy inhibition using bafilomycin A

Identifiants

DOI: 10.1007/978-1-0716-0592-9_17 PMID: 32410036 PMC: PMC9833850
pubmed: 32410036
doi: 10.1007/978-1-0716-0592-9_17
pmc: PMC9833850
mid: NIHMS1678335
doi:

Substances chimiques

Caenorhabditis elegans Proteins 0
Microtubule-Associated Proteins 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

187-200

Subventions

Organisme : NIH HHS
ID : P40 OD010440
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG028664
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG038664
Pays : United States
Organisme : NIA NIH HHS
ID : R21 AG058038
Pays : United States

Commentaires et corrections

Type : ErratumIn
Title corrected

Références

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pubmed: 17534139

Auteurs

Jessica T Chang (JT)

Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA.

Malene Hansen (M)

Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA. mhansen@sbpdiscovery.org.

Caroline Kumsta (C)

Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA. ckumsta@sbpdiscovery.org.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Vieillissement › Assessing Tissue-Specific Autophagy Flux in...
questionsmedicales.fr › Eucaryotes › Animaux › Assessing Tissue-Specific Autophagy Flux in...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Autophagie › Assessing Tissue-Specific Autophagy Flux in...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Helminthes › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Caenorhabditis › Caenorhabditis elegans › Assessing Tissue-Specific Autophagy Flux in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'helminthes › Protéines de Caenorhabditis elegans › Assessing Tissue-Specific Autophagy Flux in...
questionsmedicales.fr › Phénomènes physiologiques › Longévité › Assessing Tissue-Specific Autophagy Flux in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de tissu nerveux › Protéines associées aux microtubules › Assessing Tissue-Specific Autophagy Flux in...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Biologie moléculaire › Assessing Tissue-Specific Autophagy Flux in...