Staining and Quantification of β-Amyloid Pathology in Transgenic Mouse Models of Alzheimer's Disease.
Immunohistochemistry
Quantitative imaging
Thioflavin S
Transgenic mice
β-Amyloid
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
16
5
2020
pubmed:
16
5
2020
medline:
13
3
2021
Statut:
ppublish
Résumé
Studies of Alzheimer's disease (AD) using experimental systems most often involve transgenic mouse models that are characterized by neural accumulation of β-amyloid protein (Aβ), which is widely hypothesized to have a key role in AD pathogenesis. Quantification of Aβ in transgenic mice typically is accomplished through both biochemical and histochemical approaches. In this chapter, we describe two techniques for the histological detection of Aβ, immunostaining with Aβ antibodies and staining with the amyloid dye thioflavin S, and its quantification using digital imaging.
Identifiants
pubmed: 32410038
doi: 10.1007/978-1-0716-0592-9_19
pmc: PMC7299244
mid: NIHMS1597150
doi:
Substances chimiques
Amyloid beta-Peptides
0
Amyloid beta-Protein Precursor
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
211-221Subventions
Organisme : NIA NIH HHS
ID : RF1 AG058068
Pays : United States
Références
Neurobiol Aging. 2002 Mar-Apr;23(2):161-70
pubmed: 11804699
EMBO Mol Med. 2016 Jun 01;8(6):595-608
pubmed: 27025652
Methods Mol Biol. 2012;849:507-27
pubmed: 22528112
JAMA Neurol. 2014 Apr;71(4):505-8
pubmed: 24493463
Annu Rev Pathol. 2015;10:291-319
pubmed: 25387055
Annu Rev Neurosci. 2016 Jul 8;39:57-79
pubmed: 27050320