Rat Olfactory Mucosa Mesenchymal Stem/Stromal Cells (OM-MSCs): A Characterization Study.

Journal

International journal of cell biology
ISSN: 1687-8876
Titre abrégé: Int J Cell Biol
Pays: United States
ID NLM: 101517861

Informations de publication

Date de publication:
2020
Historique:
received: 10 05 2019
accepted: 28 09 2019
entrez: 16 5 2020
pubmed: 16 5 2020
medline: 16 5 2020
Statut: epublish

Résumé

Stem/stromal cell-based therapies are a branch of regenerative medicine and stand as an attractive option to promote the repair of damaged or dysfunctional tissues and organs. Olfactory mucosa mesenchymal stem/stromal cells have been regarded as a promising tool in regenerative therapies because of their several favorable properties such as multipotency, high proliferation rate, helpful location, and few associated ethical issues. These cells are easily accessible in the nasal cavity of most mammals, including the rat, can be easily applied in autologous treatments, and do not cope with most of the obstacles associated with the use of other stem cells. Despite this, its application in preclinical trials and in both human and animal patients is still limited because of the small number of studies performed so far and to the nonexistence of a standard and unambiguous protocol for collection, isolation, and therapeutic application. In the present work a validation of a protocol for isolation, culture, expansion, freezing, and thawing of olfactory mucosa mesenchymal stem/stromal cells was performed, applied to the rat model, as well as a biological characterization of these cells. To investigate the therapeutic potential of OM-MSCs and their eventual safe application in preclinical trials, the main characteristics of OMSC stemness were addressed.

Identifiants

DOI: 10.1155/2020/2938258 PMID: 32411249 PMC: PMC7212324
pubmed: 32411249
doi: 10.1155/2020/2938258
pmc: PMC7212324
doi:

Types de publication

Journal Article

Langues

eng

Pagination

2938258

Informations de copyright

Copyright © 2020 Rui D. Alvites et al.

Déclaration de conflit d'intérêts

The author(s) declare(s) that there are no conflicts of interest regarding the publication of this article.

Références

Methods. 2016 Apr 15;99:62-8
pubmed: 26384580
Oncol Rep. 2015 Oct;34(4):1915-22
pubmed: 26252165
J Vis Exp. 2011 Aug 22;(54):
pubmed: 21876529
Proc Natl Acad Sci U S A. 2009 Oct 13;106(41):17499-504
pubmed: 19805173
Biochimie. 2013 Dec;95(12):2212-21
pubmed: 23810910
Mol Ther Methods Clin Dev. 2016 Nov 30;3:16073
pubmed: 27990447
Acta Cir Bras. 2016 Jan;31(1):59-66
pubmed: 26840357
J Immunol Res. 2014;2014:987678
pubmed: 24741639
Rev Neurosci. 2015;26(3):281-93
pubmed: 25781675
J Biol Chem. 2000 Mar 31;275(13):9645-52
pubmed: 10734116
Ann Biomed Eng. 2017 May;45(5):1365-1374
pubmed: 28091965
Stem Cells Int. 2018 May 10;2018:2683969
pubmed: 29861741
Biosci Rep. 2015 Apr 28;35(2):
pubmed: 25797907
Neurobiol Dis. 2007 May;26(2):363-74
pubmed: 17346980
J Comp Neurol. 2015 Jan 1;523(1):15-31
pubmed: 25044230
Cell Commun Signal. 2016 Jan 12;14:2
pubmed: 26754424
Exp Mol Med. 2014 Jun 20;46:e101
pubmed: 24946789
Stem Cells. 2008 Aug;26(8):2183-92
pubmed: 18535154
Vet Immunol Immunopathol. 2011 Nov 15;144(1-2):147-54
pubmed: 21782255
BMC Vet Res. 2018 Jan 17;14(1):17
pubmed: 29343270
Vasc Health Risk Manag. 2013;9:195-206
pubmed: 23662065
Transplantation. 2012 Jul 27;94(2):132-8
pubmed: 22766769
PLoS One. 2010 Dec 07;5(12):e14247
pubmed: 21151872
Cytotherapy. 2005;7(5):393-5
pubmed: 16236628
Tissue Eng Part A. 2009 Oct;15(10):3049-59
pubmed: 19335061
Exp Hematol. 1976 Sep;4(5):267-74
pubmed: 976387
Clin Sci (Lond). 2013 Feb;124(3):165-76
pubmed: 22876972
Brain Res Dev Brain Res. 1993 Aug 20;74(2):235-44
pubmed: 8403385
Stem Cells. 2014 Jun;32(6):1380-9
pubmed: 24497003
J Exp Med. 2012 Dec 17;209(13):2351-65
pubmed: 23209317
Adv Biochem Eng Biotechnol. 2010;123:219-63
pubmed: 20309674
Front Immunol. 2017 Nov 20;8:1577
pubmed: 29209319
Stem Cells. 2006 Nov;24(11):2549-56
pubmed: 16840553
J Neurosci Res. 2012 Mar;90(3):619-31
pubmed: 22057888
Mol Ther Methods Clin Dev. 2017 Jun 23;6:102-111
pubmed: 28725658
J Neurosci. 2011 May 4;31(18):6915-27
pubmed: 21543621
Stem Cells Int. 2016;2016:9756973
pubmed: 26880998
Stem Cells Dev. 2011 May;20(5):915-23
pubmed: 20879854
Stem Cells Dev. 2010 Jun;19(6):853-66
pubmed: 19905894
PLoS One. 2009 Jun 09;4(6):e5846
pubmed: 19513108
J Peripher Nerv Syst. 2007 Jun;12(2):65-82
pubmed: 17565531
Biochem Biophys Res Commun. 2008 Nov 14;376(2):419-22
pubmed: 18789891
Stem Cells Dev. 2014 Mar 1;23(5):502-14
pubmed: 24172073
Stem Cells Dev. 2011 Dec;20(12):2077-91
pubmed: 21401444
Int J Stem Cells. 2014 Nov;7(2):118-26
pubmed: 25473449
Am J Hematol. 2007 Jun;82(6):463-72
pubmed: 17266054
Int J Stem Cells. 2014 Nov;7(2):135-42
pubmed: 25473451
Stem Cells Int. 2018 Aug 19;2018:8031718
pubmed: 30210552
Stem Cells Transl Med. 2017 Oct;6(10):1894-1904
pubmed: 28960910
J Zhejiang Univ Sci B. 2009 May;10(5):355-67
pubmed: 19434762
J Tissue Eng Regen Med. 2015 Dec;9(12):E191-201
pubmed: 23378029
Mol Med Rep. 2015 Jul;12(1):20-30
pubmed: 25739039
Stem Cells Dev. 2012 Oct 10;21(15):2789-97
pubmed: 22533467
Biopreserv Biobank. 2015 Aug;13(4):231-9
pubmed: 26280501
Oncotarget. 2015 Jun 30;6(18):15966-83
pubmed: 25965826
Gene. 2016 Aug 1;587(1):1-17
pubmed: 27129941
Clin Biochem. 2004 Jul;37(7):529-40
pubmed: 15234234
Int J Mol Sci. 2016 Jul 19;17(7):
pubmed: 27447618
Transfus Med Hemother. 2011;38(2):107-123
pubmed: 21566712
Anat Rec (Hoboken). 2018 Oct;301(10):1678-1689
pubmed: 29710430
Sci Rep. 2019 Feb 11;9(1):1774
pubmed: 30742027
Stem Cells Transl Med. 2017 Dec;6(12):2146-2159
pubmed: 29090533
Physiol Rev. 2012 Oct;92(4):1619-49
pubmed: 23073628
Int Rev Cytol. 1984;89:151-77
pubmed: 6206019
Brain Res. 2005 May 31;1045(1-2):45-56
pubmed: 15885668
Acta Biomater. 2015 Feb;13:266-76
pubmed: 25463488
Curr Protoc Immunol. 2015 Nov 02;111:A3.B.1-A3.B.3
pubmed: 26529666
Stem Cells. 2009 Sep;27(9):2196-208
pubmed: 19544421
Stem Cell Res Ther. 2014 Jul 07;5(4):85
pubmed: 25157428
Methods Mol Biol. 2013;1059:107-14
pubmed: 23934838
Cells Tissues Organs. 2018;206(1-2):35-45
pubmed: 30630170
Injury. 2014 Oct;45 Suppl 4:S2-6
pubmed: 25384470
J Cell Physiol. 2007 Sep;212(3):702-9
pubmed: 17477371
Arthritis Rheum. 2005 Aug;52(8):2521-9
pubmed: 16052568
Cancer Res. 2013 Sep 1;73(17):5347-59
pubmed: 23838935
Vet Surg. 2018 Jan;47(1):19-29
pubmed: 29023790
Cytotherapy. 2006;8(4):315-7
pubmed: 16923606
Stem Cell Res. 2015 Jul;15(1):23-9
pubmed: 25965912
Differentiation. 2014 Mar-Apr;87(3-4):147-60
pubmed: 24713343
Immunology. 1992 Aug;76(4):560-5
pubmed: 1328039
Acta Orthop Scand. 1983 Oct;54(5):743-5
pubmed: 6670492
ScientificWorldJournal. 2010 Mar 16;10:422-33
pubmed: 20305985
BMC Cell Biol. 2006 Mar 10;7:14
pubmed: 16529651
Mol Med Rep. 2018 Jan;17(1):1667-1675
pubmed: 29138837
BMC Vet Res. 2011 Aug 02;7:42
pubmed: 21810270
Cell Death Differ. 2014 Feb;21(2):216-25
pubmed: 24185619
Methods Mol Biol. 2016;1416:159-69
pubmed: 27236671
J Vet Sci. 2012 Sep;13(3):299-310
pubmed: 23000587
Cytometry A. 2014 Aug;85(8):678-87
pubmed: 24894974
Regen Med. 2014;9(5):649-72
pubmed: 25372080
Regen Med. 2018 Jul 1;13(5):581-593
pubmed: 30113240
World J Stem Cells. 2010 Aug 26;2(4):67-80
pubmed: 21607123
BMC Vet Res. 2014 Oct 08;10:228
pubmed: 25293656
Cell. 1983 Feb;32(2):627-38
pubmed: 6130854
J Neurosci. 1986 Jan;6(1):208-17
pubmed: 3944619
Int Forum Allergy Rhinol. 2013 Jul;3(7):550-5
pubmed: 23423973
Osteoarthritis Cartilage. 2007 Jun;15(6):646-55
pubmed: 17337215
J Vet Sci. 2009 Sep;10(3):181-7
pubmed: 19687617
J Clin Invest. 2011 Jul;121(7):2808-20
pubmed: 21670501
Trends Immunol. 2012 Mar;33(3):136-43
pubmed: 22227317
Am J Clin Pathol. 2015 Feb;143(2):177-85; quiz 305
pubmed: 25596243
Tissue Eng Part B Rev. 2017 Oct;23(5):462-470
pubmed: 28142381
Glia. 2013 Mar;61(3):368-82
pubmed: 23281012
Sci Justice. 2006 Jul-Sep;46(3):179-84
pubmed: 17388245
Anticancer Res. 1998 Nov-Dec;18(6B):4705-8
pubmed: 9891544
Cytotherapy. 2012 Nov;14(10):1203-14
pubmed: 22909277
PLoS One. 2017 Jul 18;12(7):e0181151
pubmed: 28719644
Biomed Res Int. 2014;2014:302659
pubmed: 25121094
Trends Neurosci. 2015 Jun;38(6):364-74
pubmed: 25975510
Biomed Res Int. 2017;2017:7407168
pubmed: 28573141
Stem Cells. 2011 Apr;29(4):670-7
pubmed: 21312317
Cell Mol Immunol. 2016 May;13(3):401-8
pubmed: 26388237
Dev Growth Differ. 2013 Jun;55(5):591-605
pubmed: 23557080
J Cell Mol Med. 2013 Sep;17(9):1136-45
pubmed: 23802625
Clin Otolaryngol Allied Sci. 1990 Dec;15(6):553-8
pubmed: 2073765
Biomaterials. 2012 Mar;33(7):2086-96
pubmed: 22169138
Front Immunol. 2018 Dec 04;9:2837
pubmed: 30564236
J Transl Med. 2014 Oct 11;12:260
pubmed: 25304688
Sci Rep. 2018 Jun 18;8(1):9321
pubmed: 29915318
Stem Cell Res. 2014 May;12(3):673-9
pubmed: 24681208
Stem Cells Int. 2016;2016:1243659
pubmed: 26949398
Methods Mol Biol. 2013;1048:1-10
pubmed: 23929093
Stem Cells Int. 2018 Oct 8;2018:8179075
pubmed: 30402112
Cytometry A. 2012 Apr;81(4):312-23
pubmed: 22411893
Exp Ther Med. 2015 Apr;9(4):1192-1200
pubmed: 25780408
BMC Cell Biol. 2014 Dec 20;15:46
pubmed: 25526965

Auteurs

Rui D Alvites (RD)

Departamento de Clínicas Veterinárias, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal.
Centro de Estudos de Ciência Animal (CECA), Instituto de Ciências, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal.
ORCID: 0000-0003-1945-6377

Mariana V Branquinho (MV)

Departamento de Clínicas Veterinárias, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal.
Centro de Estudos de Ciência Animal (CECA), Instituto de Ciências, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal.
ORCID: 0000-0003-1328-464X

Ana R Caseiro (AR)

Departamento de Clínicas Veterinárias, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal.
Centro de Estudos de Ciência Animal (CECA), Instituto de Ciências, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal.
REQUIMTE/LAQV - U. Porto - Porto/Portugal, Departamento de Engenharia Metalúrgica e Materiais, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal.
Escola Universitária Vasco da Gama (EUVG), Avenida José R. Sousa Fernandes, nº 197 Lordemão, 3020-210 Coimbra, Portugal.

Irina Amorim (I)

Departamento de Patologia e Imunologia Molecular, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal.
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, R. Alfredo Allen, 4200-135 Porto, Portugal.
Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), 4200-465 Porto, Portugal.
ORCID: 0000-0002-2079-216X

Sílvia Santos Pedrosa (S)

Departamento de Clínicas Veterinárias, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal.
Centro de Estudos de Ciência Animal (CECA), Instituto de Ciências, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal.
ORCID: 0000-0002-2055-8031

Alexandra Rêma (A)

Departamento de Patologia e Imunologia Molecular, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal.
ORCID: 0000-0003-4319-2404

Fátima Faria (F)

Departamento de Patologia e Imunologia Molecular, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal.

Beatriz Porto (B)

Laboratório de Citogenética, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal.
ORCID: 0000-0003-4281-5438

Cláudia Oliveira (C)

Laboratório de Citogenética, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal.
ORCID: 0000-0003-0556-406X

Paula Teixeira (P)

Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital 172, 4200-374 Porto, Portugal.
ORCID: 0000-0002-6296-5137

Rui Magalhães (R)

Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital 172, 4200-374 Porto, Portugal.
ORCID: 0000-0003-3172-440X

Stefano Geuna (S)

Department of Clinical and Biological Sciences, and Cavalieri Ottolenghi Neuroscience Institute, University of Turin, Ospedale San Luigi, 10043 Orbassano, Turin, Italy.
ORCID: 0000-0002-6962-831X

Artur S P Varejão (ASP)

Departamento de Ciências Veterinárias, Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal.
CECAV, Centro de Ciência Animal e Veterinária, Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal.
ORCID: 0000-0002-0736-3635

Ana C Maurício (AC)

Departamento de Clínicas Veterinárias, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal.
Centro de Estudos de Ciência Animal (CECA), Instituto de Ciências, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal.
ORCID: 0000-0002-0018-9363

Classifications MeSH