17Beta-Estradiol Regulates NUCB2/ Nesfatin-1 Expression in Mouse Oviduct.
17beta-estradiol
Gonadotropin
NUCB2/nesfatin-1
Oviduct
Journal
Development & reproduction
ISSN: 2465-9525
Titre abrégé: Dev Reprod
Pays: Korea (South)
ID NLM: 101178352
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
13
01
2020
revised:
21
01
2020
accepted:
22
01
2020
entrez:
16
5
2020
pubmed:
16
5
2020
medline:
16
5
2020
Statut:
ppublish
Résumé
NUCB2/nesfatin-1 known to regulate appetite and energy homeostasis is expressed not only in the hypothalamus, but also in various organs and tissues. Our previous reports also demonstrated that NUCB2/nesfatin-1 was expressed in the reproductive organs, including the ovaries, uterus, and testes of mice. However, it is yet known whether NUCB2/nesfatin-1 is expressed in the oviduct and how its expression is regulated. Therefore, we investigated the expression of NUCB2/nesfatin-1 in the oviduct and its expression is regulated by gonadotropin. Immunohistochemical staining results showed that nesfatin-1 protein was localized in epithelial cells of the oviduct. As a result of quantitative real-time PCR (qRT-PCR) and Western blot, NUCB2/nesfatin-1 was detected strongly in the oviducts. During the estrus cycle, NUCB2/nesfatin-1 expression in the oviducts was markedly higher in the proestrus stage than in other estrus stages. In order to elucidate whether the expression of NUCB2 mRNA is controlled by the gonadotropins, we injected PMSG and hCG and measured NUCB2 mRNA level in the oviduct after injection. Its level was increased in the oviduct after PMSG injection, but no significant change after hCG injection. In addition, NUCB2 mRNA levels were markedly reduced after ovariectomy, while recovered after 17β-estradiol (E2) injection, but not by progesterone (P4). This study demonstrated that NUCB2/nesfatin-1 is highly expressed in the oviduct of mouse and its expression is regulated by E2 secreted by the ovaries. These results suggest that NUCB2/nesfatin-1 expressed by the oviduct may affect the function of the oviduct regulated by the ovaries.
Identifiants
pubmed: 32411917
doi: 10.12717/DR.2020.24.1.43
pii: dr-24-1-43
pmc: PMC7201062
doi:
Types de publication
Journal Article
Langues
eng
Pagination
43-52Informations de copyright
© Copyright 2020 The Korean Society of Developmental Biology.
Déclaration de conflit d'intérêts
The authors declare no potential conflict of interest.
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