Touch DNA in forensic science: The use of laboratory-created eccrine fingerprints to quantify DNA loss.
DNA loss
Fingerprint
Forensic
Touch DNA
Journal
Forensic science international. Synergy
ISSN: 2589-871X
Titre abrégé: Forensic Sci Int Synerg
Pays: Netherlands
ID NLM: 101766849
Informations de publication
Date de publication:
2020
2020
Historique:
received:
12
08
2019
revised:
17
10
2019
accepted:
18
10
2019
entrez:
16
5
2020
pubmed:
16
5
2020
medline:
16
5
2020
Statut:
epublish
Résumé
Touch samples typically contain a limited quantity of DNA, which can be further reduced during collection and analysis. It is not clear, however, at which point(s) the majority of the DNA is lost because there is not a reliable positive control to track the quantity of DNA through the analysis procedures. To take the first step in bridging this gap, we established a set of laboratory-created eccrine, or mock, fingerprints containing known quantities of DNA. Next, we defined a set of process controls to quantify loss at key fail points in the collection/extraction procedures, analyzing a total of 1200 mock fingerprints deposited on four different surfaces. We quantified DNA loss to the surface, the swab and at extraction, completing the evaluation with ANOVA. With better understanding of DNA yields and the mechanisms of loss, targeted process improvements will bring touch DNA samples into even more routine use with standardized, optimized procedures.
Identifiants
pubmed: 32411992
doi: 10.1016/j.fsisyn.2019.10.004
pii: S2589-871X(19)30157-3
pmc: PMC7219128
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1-16Informations de copyright
© 2019 The Authors.
Déclaration de conflit d'intérêts
None.
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