SARS-CoV-2 infection diagnosed only by cell culture isolation before the local outbreak in an Italian seven-week-old suckling baby.
COVID-19
Cell culture
Diagnosis
SARS-CoV-2
Suckling baby
Journal
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
ISSN: 1878-3511
Titre abrégé: Int J Infect Dis
Pays: Canada
ID NLM: 9610933
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
06
04
2020
revised:
05
05
2020
accepted:
09
05
2020
pubmed:
18
5
2020
medline:
29
7
2020
entrez:
18
5
2020
Statut:
ppublish
Résumé
SARS-CoV-2 emerged in China in December 2019 and has now been declared a pandemic by the World Health Organization. This paper described the case of a 7-week-old suckling baby from Italy who was SARS-CoV-2-positive only by the cell culture method, with no clinical suspicion of and/or risk factors for SARS-CoV-2 infection. The baby was referred to hospital, with signs and symptoms of upper respiratory tract infection, before the virus had spread to the province. Nasal and pharyngeal swabs and a nasopharyngeal aspirate were used for conventional and molecular diagnostic assays not including the SARS-CoV-2 virus. Bacteria referred to the resident population were revealed in nasal and pharyngeal swabs. No viruses were detected using both immunofluorescence assay and nucleic acid amplification assays in the nasopharyngeal aspirate. The baby was discharged in good condition after 3 days of hospitalisation. Later, a cytopathic effect on the cell monolayers currently used for respiratory viruses was observed and the viral particles were identified as Coronaviridae by transmission electron microscopy. SARS-CoV-2 was identified by RT-PCR performed both on cell culture and on the stored aliquot of the original sample. The virus isolate was named SARS-Cov-2/human/Parma/1/2020. Cell culture still remains the only reference diagnostic method also for emerging viruses, allowing it to reveal cytopathogenic viruses and demonstrate their infectivity.
Identifiants
pubmed: 32417248
pii: S1201-9712(20)30342-8
doi: 10.1016/j.ijid.2020.05.035
pmc: PMC7224665
pii:
doi:
Types de publication
Case Reports
Langues
eng
Sous-ensembles de citation
IM
Pagination
387-389Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.
Références
Clin Infect Dis. 2020 Jul 28;71(15):853-857
pubmed: 32161941
Euro Surveill. 2020 Mar;25(9):
pubmed: 32156327
Sci Rep. 2014 Oct 30;4:6803
pubmed: 25354905
Glob Health Res Policy. 2020 Mar 2;5:7
pubmed: 32158961
J Med Virol. 2020 Jun;92(6):564-567
pubmed: 32115733
Lancet. 2020 Feb 15;395(10223):497-506
pubmed: 31986264
AJR Am J Roentgenol. 2020 Jul;215(1):87-93
pubmed: 32174129
Diagn Microbiol Infect Dis. 2019 Jul;94(3):260-267
pubmed: 30745224
Sci Rep. 2016 Oct 27;6:36082
pubmed: 27786297
Nat Microbiol. 2020 Apr;5(4):536-544
pubmed: 32123347