A versatile and modular tetrode-based device for single-unit recordings in rodent ex vivo and in vivo acute preparations.
Ex vivo brain slices
Extracellular electrophysiology
In vivo auditory cortex
Rodent recordings
Tetrodes
Journal
Journal of neuroscience methods
ISSN: 1872-678X
Titre abrégé: J Neurosci Methods
Pays: Netherlands
ID NLM: 7905558
Informations de publication
Date de publication:
15 07 2020
15 07 2020
Historique:
received:
11
02
2020
revised:
05
04
2020
accepted:
28
04
2020
pubmed:
18
5
2020
medline:
22
6
2021
entrez:
18
5
2020
Statut:
ppublish
Résumé
The demand for affordable tools for recording extracellular activity and successfully isolating single units from different brain preparations has pushed researchers and companies to invest in developing and fabricating new recording devices. However, depending on the brain region of interest, experimental question or type of preparation, different devices are required thus adding substantial financial burden to laboratories. We have developed a simple and affordable tetrode-based device that allows interchangeable extracellular recordings of neuronal activity between in vivo and ex vivo preparations and can be easily implemented in all wet-bench laboratories. Spontaneous activity from several putative single neurons could be easily recorded and isolated by lowering the device into ex vivo cerebellum brain slices. The same device was also used in vivo, lowered into primary auditory cortex of adult anesthetized transgenic mice expressing channelrhodopsin in cortical neurons. Acoustic stimulation of the contralateral ear or direct laser optogenetic stimulation successfully evoked cortical activity at the recording site. Several isolated putative single neurons presented time-locked activity response to the different stimuli. Besides low fabrication cost, our device uses an omnetics connector compatible with the majority of headstages already available at most electrophysiology laboratories. The device allows custom tetrode configuration arrays and extensions for optogenetics and pharmacology, providing experimental flexibility not available in commercial off-the-shelf microelectrode arrays and silicon probes. We developed an affordable, versatile and modular device to facilitate tetrode extracellular recordings interchangeably between in vivo anaesthetized animals and ex vivo brain slice recordings.
Sections du résumé
BACKGROUND
The demand for affordable tools for recording extracellular activity and successfully isolating single units from different brain preparations has pushed researchers and companies to invest in developing and fabricating new recording devices. However, depending on the brain region of interest, experimental question or type of preparation, different devices are required thus adding substantial financial burden to laboratories.
NEW METHOD
We have developed a simple and affordable tetrode-based device that allows interchangeable extracellular recordings of neuronal activity between in vivo and ex vivo preparations and can be easily implemented in all wet-bench laboratories.
RESULTS
Spontaneous activity from several putative single neurons could be easily recorded and isolated by lowering the device into ex vivo cerebellum brain slices. The same device was also used in vivo, lowered into primary auditory cortex of adult anesthetized transgenic mice expressing channelrhodopsin in cortical neurons. Acoustic stimulation of the contralateral ear or direct laser optogenetic stimulation successfully evoked cortical activity at the recording site. Several isolated putative single neurons presented time-locked activity response to the different stimuli.
COMPARISON WITH EXISTING METHODS
Besides low fabrication cost, our device uses an omnetics connector compatible with the majority of headstages already available at most electrophysiology laboratories. The device allows custom tetrode configuration arrays and extensions for optogenetics and pharmacology, providing experimental flexibility not available in commercial off-the-shelf microelectrode arrays and silicon probes.
CONCLUSIONS
We developed an affordable, versatile and modular device to facilitate tetrode extracellular recordings interchangeably between in vivo anaesthetized animals and ex vivo brain slice recordings.
Identifiants
pubmed: 32417534
pii: S0165-0270(20)30178-3
doi: 10.1016/j.jneumeth.2020.108755
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108755Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.