Role of betaine in inhibiting the induction of RNA Pol III gene transcription and cell growth caused by alcohol.
Alcohol
Betaine
Brf1
Cell growth
Colony formation
Pol III genes
Journal
Chemico-biological interactions
ISSN: 1872-7786
Titre abrégé: Chem Biol Interact
Pays: Ireland
ID NLM: 0227276
Informations de publication
Date de publication:
01 Jul 2020
01 Jul 2020
Historique:
received:
06
06
2019
revised:
24
03
2020
accepted:
06
05
2020
pubmed:
19
5
2020
medline:
17
6
2020
entrez:
19
5
2020
Statut:
ppublish
Résumé
Alcohol has been classified as carcinogenic to humans by the International Agency for Research on Cancer (IARC). Studies have demonstrated that alcohol intake increases the risk of breast cancer, and alcohol also stimulates breast cancer cell growth. Deregulation of Pol III genes is tightly associated with tumour development. Transcription factor II-B (TFIIB)-related factor 1 (Brf1) is a transcription factor that specifically regulates Pol III gene transcription. Our in vivo and in vitro studies have indicated that alcohol enhances the transcription of Pol III genes to cause an alteration of cellular phenotypes, which is closely related with human breast cancer. Betaine is a vegetable alkaloid and has antitumor functions. Most reports about betaine show that the consumption level of betaine is inversely associated with a risk of breast cancer. Although different mechanisms of betaine against tumour have been investigated, nothing has been reported on the effect of betaine on the deregulation of Brf1 and Pol III genes. In this study, we determine the role of betaine in breast cancer cell growth and colony formation and explore its mechanism. Our results indicate that alcohol increases the rates of growth and colony formation of breast cancer cells, whereas betaine is able to significantly inhibit the effects of alcohol on these cell phenotypes. Betaine decreases the induction of Brf1 expression and Pol III gene transcription caused by ethanol to reduce the rates of cell growth and colony formation. Together, these studies provide novel insights into the role of betaine in alcohol-caused breast cancer cell growth and deregulation of Brf1 and Pol III genes. These results suggest that betaine consumption is able to prevent alcohol-associated human cancer development.
Identifiants
pubmed: 32418914
pii: S0009-2797(19)30992-5
doi: 10.1016/j.cbi.2020.109129
pmc: PMC7323736
mid: NIHMS1594809
pii:
doi:
Substances chimiques
Ethanol
3K9958V90M
Betaine
3SCV180C9W
RNA Polymerase II
EC 2.7.7.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109129Subventions
Organisme : NIAAA NIH HHS
ID : R01 AA024169
Pays : United States
Organisme : NIAAA NIH HHS
ID : R13 AA027725
Pays : United States
Organisme : NIAAA NIH HHS
ID : R21 AA021114
Pays : United States
Organisme : NIAAA NIH HHS
ID : R21 AA023247
Pays : United States
Informations de copyright
Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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