Novel Non-integrating DNA Nano-S/MAR Vectors Restore Gene Function in Isogenic Patient-Derived Pancreatic Tumor Models.
S/MAR
antibiotic-free
gene supplementation
isogenic cells
nano-DNA vector
non-integrating
tumor models
Journal
Molecular therapy. Methods & clinical development
ISSN: 2329-0501
Titre abrégé: Mol Ther Methods Clin Dev
Pays: United States
ID NLM: 101624857
Informations de publication
Date de publication:
12 Jun 2020
12 Jun 2020
Historique:
received:
31
03
2020
accepted:
22
04
2020
entrez:
19
5
2020
pubmed:
19
5
2020
medline:
19
5
2020
Statut:
epublish
Résumé
We describe herein non-integrating minimally sized nano-S/MAR DNA vectors, which can be used to genetically modify dividing cells in place of integrating vectors. They represent a unique genetic tool, which avoids vector-mediated damage. Previous work has shown that DNA vectors comprising a mammalian S/MAR element can provide persistent mitotic stability over hundreds of cell divisions, resisting epigenetic silencing and thereby allowing sustained transgene expression. The composition of the original S/MAR vectors does present some inherent limitations that can provoke cellular toxicity. Herein, we present a new system, the nano-S/MAR, which drives higher transgene expression and has improved efficiency of establishment,
Identifiants
pubmed: 32420409
doi: 10.1016/j.omtm.2020.04.017
pii: S2329-0501(20)30077-2
pmc: PMC7218229
doi:
Types de publication
Journal Article
Langues
eng
Pagination
957-968Informations de copyright
© 2020 The Authors.
Références
PLoS One. 2012;7(10):e47920
pubmed: 23110132
J Biol Chem. 2000 Sep 22;275(38):29244-56
pubmed: 10878024
BMC Cell Biol. 2007 Aug 06;8:33
pubmed: 17683605
Gene Ther. 2008 Dec;15(24):1593-605
pubmed: 18633447
J Biol Chem. 2001 Jun 22;276(25):23018-27
pubmed: 11304530
Oncotarget. 2015 Jun 30;6(18):15966-83
pubmed: 25965826
Pancreas. 2010 May;39(4):425-35
pubmed: 20418756
World J Gastroenterol. 2012 Mar 28;18(12):1286-94
pubmed: 22493542
Gene Ther. 2011 Jan;18(1):82-7
pubmed: 20739959
Clin Exp Metastasis. 2005;22(6):461-73
pubmed: 16320109
Tumour Biol. 2015 Jan;36(1):111-9
pubmed: 25464861
Nat Med. 2016 Mar;22(3):278-87
pubmed: 26855150
Ann Oncol. 2015 Apr;26(4):779-86
pubmed: 25623049
J Mol Med (Berl). 2011 May;89(5):515-29
pubmed: 21301798
Mol Endocrinol. 2015 Oct;29(10):1440-53
pubmed: 26284758
Nucleic Acids Res. 2002 Jan 1;30(1):207-10
pubmed: 11752295
Chromosome Res. 2003;11(5):413-21
pubmed: 12971718
Science. 1992 Jan 10;255(5041):195-7
pubmed: 1553545
Cold Spring Harb Protoc. 2012 Jun 01;2012(6):706-8
pubmed: 22661431
Cancer Res. 2014 Jun 1;74(11):2913-21
pubmed: 24840647
Nucleic Acids Res. 1999 Jan 15;27(2):426-8
pubmed: 9862961
J Biol Chem. 2001 Oct 19;276(42):38502-10
pubmed: 11502752
Pancreas. 2017 Mar;46(3):311-322
pubmed: 27846146
Gene. 2010 Oct 15;466(1-2):36-42
pubmed: 20621169
J Neurosurg. 2007 Sep;107(3):610-6
pubmed: 17886562
Fibrogenesis Tissue Repair. 2010 Jan 05;3(1):2
pubmed: 20051102
Future Oncol. 2016 Aug;12(16):1929-46
pubmed: 27246628
Biotechnol Adv. 2009 Jul-Aug;27(4):353-70
pubmed: 19233255
Biotechnol Bioeng. 2009 Oct 15;104(3):505-15
pubmed: 19557837
Gene Ther. 2011 Apr;18(4):334-43
pubmed: 21107439
Cell. 2016 Feb 25;164(5):1015-30
pubmed: 26898331
N Engl J Med. 2014 Nov 27;371(22):2140-1
pubmed: 25427123
J Cell Sci. 2010 Dec 15;123(Pt 24):4231-9
pubmed: 21081648
Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):6733-8
pubmed: 9618481
Expert Opin Biol Ther. 2011 Sep;11(9):1177-91
pubmed: 21548848
Vaccine. 2009 Oct 30;27(46):6454-9
pubmed: 19559109