Ethanol extract of Pycnoporus sanguineus relieves the dextran sulfate sodium-induced experimental colitis by suppressing helper T cell-mediated inflammation via apoptosis induction.
Animals
Anti-Inflammatory Agents
/ isolation & purification
Apoptosis
/ drug effects
Colitis
/ drug therapy
Cytokines
/ metabolism
Dextran Sulfate
/ toxicity
Disease Models, Animal
Ethanol
/ chemistry
Inflammation
/ drug therapy
Inflammatory Bowel Diseases
/ drug therapy
Male
Mice
Mice, Inbred BALB C
Plant Extracts
/ pharmacology
Polyporaceae
/ chemistry
T-Lymphocytes, Helper-Inducer
/ immunology
Apoptosis
Helper T cells
Inflammatory bowel disease
Pycnoporus sanguineus
Journal
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
ISSN: 1950-6007
Titre abrégé: Biomed Pharmacother
Pays: France
ID NLM: 8213295
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
04
02
2020
revised:
18
04
2020
accepted:
28
04
2020
pubmed:
19
5
2020
medline:
12
2
2021
entrez:
19
5
2020
Statut:
ppublish
Résumé
Inflammatory bowel disease (IBD) is a chronic relapsing inflammation involving the gut system, and disequilibrium of T helper (Th) cell paradigm has been recognized as critical pathogenesis. Pycnoporus sanguineus (L.) Murrill is a species of the white-rot basidiomycetes listed as food- and cosmetic-grade microorganisms. In this study, anti-inflammatory activity of the ethanol extract from P. sanguineus (PSE) was investigated in dextran sulfate sodium (DSS)-induced experimental colitis model. PSE recovered the DSS-caused weight loss, reversed the colon shortening, and ameliorated the histopathological lesion in colon, resulting in lower disease activity index (DAI). Levels of serumal lipopolysaccharide (LPS), colonic myeloperoxidase (MPO) in the colitis-suffering mice were declined by PSE treatment. PSE also improved the mucosal integrity by enhancing the expression of tight junction and adherens junction proteins in the colon, including ZO-1, occludin, claudin-1, and E-cadherin. Besides, PSE reduced helper T cells (Th) in the colon, together with an evident decrease of several Th cell-related cytokines. Moreover, it was found that in vitro, PSE suppressed T cells and the Th subset upon Concanavalin A (ConA)-stimulation by inducing apoptosis. In summary, PSE displayed a remission on the colitis-related inflammation, which would possibly rely on the epithelial barrier restoration by suppressing Th cells via apoptosis induction, highlighting a promising potential in the treatment for IBD.
Identifiants
pubmed: 32422567
pii: S0753-3322(20)30404-2
doi: 10.1016/j.biopha.2020.110212
pii:
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Cytokines
0
Plant Extracts
0
Ethanol
3K9958V90M
Dextran Sulfate
9042-14-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
110212Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Masson SAS.. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest Author Shunxian Li and Yizhen Xie were employed by Guangdong Yuewei Edible Fungi Technology Co. Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.