Quantitative Proteomics Identifies TCF1 as a Negative Regulator of Foxp3 Expression in Conventional T Cells.
Immunology
Molecular Biology
Molecular Mechanism of Gene Regulation
Proteomics
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
22 May 2020
22 May 2020
Historique:
received:
03
03
2020
revised:
02
04
2020
accepted:
29
04
2020
pubmed:
19
5
2020
medline:
19
5
2020
entrez:
19
5
2020
Statut:
ppublish
Résumé
Regulatory T cells are important regulators of the immune system and have versatile functions for the homeostasis and repair of tissues. They express the forkhead box transcription factor Foxp3 as a lineage-defining protein. Negative regulators of Foxp3 expression are not well understood. Here, we generated double-stranded DNA probes complementary to the Foxp3 promoter sequence and performed a pull-down with nuclear protein in vitro, followed by elution of bound proteins and quantitative mass spectrometry. Of the Foxp3-promoter-binding transcription factors identified with this approach, one was T cell factor 1 (TCF1). Using viral over-expression, we identified TCF1 as a repressor of Foxp3 expression. In TCF1-deficient animals, increased levels of Foxp3
Identifiants
pubmed: 32422593
pii: S2589-0042(20)30312-6
doi: 10.1016/j.isci.2020.101127
pmc: PMC7229326
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
101127Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing financial interests.
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