IL-15 Expression Pattern in Atopic Dermatitis.

Biomarkers / metabolism Case-Control Studies Dermatitis, Atopic / diagnosis Humans Interleukin-15 / metabolism Skin / immunology
Atopic dermatitis Dendritic cell Fibroblast Interleukin 15 Keratinocyte

Journal

International archives of allergy and immunology
ISSN: 1423-0097
Titre abrégé: Int Arch Allergy Immunol
Pays: Switzerland
ID NLM: 9211652

Informations de publication

Date de publication:
2020
Historique:
received: 03 05 2020
accepted: 04 05 2020
pubmed: 19 5 2020
medline: 11 11 2020
entrez: 19 5 2020
Statut: ppublish

Résumé

An increased expression of interleukin (IL)-15, a cytokine with a key role in stimulating innate and adaptive immune cells, such as dendritic cells (DC), natural killer cells, and T cells, has been observed in infectious and inflammatory diseases, including autoimmune diseases as well as cancer. Atopic dermatitis (AD) is a common inflammatory skin disease characterized by a type 2 immune response. To explore the expression of IL-15 and its pattern in AD skin. Immunofluorescence staining was performed on skin specimens of AD skin, nonlesional AD skin (AD NL), and normal skin (NS) using antibodies directed against IL-15 and CD3, mast cell tryptase, eosinophil cationic protein, CD68, CD11b, CD1a, and vimentin. A significantly higher IL-15 expression in AD and AD NL was observed in both the epidermis (p = 0.0003) and the dermis (p = 0.0154) as compared to NS. Cells expressing IL-15 were mainly keratinocytes, CD1a+ DC, CD11b+ DC, CD68+ macrophages, and vimentin+ fibroblasts. In AD, an increase in the relative numbers of IL-15 expressing CD1a+ DC, macrophages, and fibroblasts was noted. Our results demonstrate an expression of IL-15 in AD similar to that of eosinophilic esophagitis which is also a type 2 disease. IL-15 may serve as a therapeutic target for AD.

Sections du résumé

BACKGROUND
An increased expression of interleukin (IL)-15, a cytokine with a key role in stimulating innate and adaptive immune cells, such as dendritic cells (DC), natural killer cells, and T cells, has been observed in infectious and inflammatory diseases, including autoimmune diseases as well as cancer. Atopic dermatitis (AD) is a common inflammatory skin disease characterized by a type 2 immune response.
OBJECTIVE
To explore the expression of IL-15 and its pattern in AD skin.
METHOD
Immunofluorescence staining was performed on skin specimens of AD skin, nonlesional AD skin (AD NL), and normal skin (NS) using antibodies directed against IL-15 and CD3, mast cell tryptase, eosinophil cationic protein, CD68, CD11b, CD1a, and vimentin.
RESULTS
A significantly higher IL-15 expression in AD and AD NL was observed in both the epidermis (p = 0.0003) and the dermis (p = 0.0154) as compared to NS. Cells expressing IL-15 were mainly keratinocytes, CD1a+ DC, CD11b+ DC, CD68+ macrophages, and vimentin+ fibroblasts. In AD, an increase in the relative numbers of IL-15 expressing CD1a+ DC, macrophages, and fibroblasts was noted.
CONCLUSION
Our results demonstrate an expression of IL-15 in AD similar to that of eosinophilic esophagitis which is also a type 2 disease. IL-15 may serve as a therapeutic target for AD.

Identifiants

DOI: 10.1159/000508515 PMID: 32422638
pubmed: 32422638
pii: 000508515
doi: 10.1159/000508515
doi:

Substances chimiques

Biomarkers 0
IL15 protein, human 0
Interleukin-15 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

417-421

Informations de copyright

© 2020 S. Karger AG, Basel.

Auteurs

Hélène Karlen (H)

Department of Dermatology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.

Shida Yousefi (S)

Institute of Pharmacology, University of Bern, Bern, Switzerland.

Hans-Uwe Simon (HU)

Institute of Pharmacology, University of Bern, Bern, Switzerland.
Department of Clinical Immunology and Allergology, Sechenov University, Moscow, Russian Federation.

Dagmar Simon (D)

Department of Dermatology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland, dagmar.simon@insel.ch.

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Classifications MeSH

questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › IL-15 Expression Pattern in Atopic Dermatitis.
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Caractéristiques des études épidémiologiques › Études épidémiologiques › Études cas-témoins › IL-15 Expression Pattern in Atopic Dermatitis.
questionsmedicales.fr › Maladies du système immunitaire › Hypersensibilité › Hypersensibilité immédiate › Eczéma atopique › IL-15 Expression Pattern in Atopic Dermatitis.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › IL-15 Expression Pattern in Atopic Dermatitis.
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Cytokines › Interleukines › Interleukine-15 › IL-15 Expression Pattern in Atopic Dermatitis.
questionsmedicales.fr › Système tégumentaire › Peau › IL-15 Expression Pattern in Atopic Dermatitis.