The Potential Risk of Plant-Virus Disease Initiation by Infected Tomatoes.
mechanical transmission
pepino mosaic virus
primary viral inoculum
tomato brown rugose fruit virus
Journal
Plants (Basel, Switzerland)
ISSN: 2223-7747
Titre abrégé: Plants (Basel)
Pays: Switzerland
ID NLM: 101596181
Informations de publication
Date de publication:
14 May 2020
14 May 2020
Historique:
received:
01
04
2020
revised:
07
05
2020
accepted:
11
05
2020
entrez:
20
5
2020
pubmed:
20
5
2020
medline:
20
5
2020
Statut:
epublish
Résumé
During 2019, tomato fruits showing viral-like symptoms of marbled yellow spots were abundant in Israel. The new symptoms were distinctive from those typical of tomato brown rugose fruit virus (ToBRFV) infection but resembled symptoms of pepino mosaic virus (PepMV) infection. RT-PCR analysis and the serological tests (enzyme linked immunosorbent assay, western blot and in situ immunofluorescence) revealed and confirmed the presence of both the tobamovirus ToBRFV and the potexvirus PepMV in the symptomatic fruits. A mixture of rod-like and filamentous particles, characteristic of viruses belonging to tobamovirus and potexvirus genera, was visualized by transmission electron microscopy of the tomato fruit viral extract. Sanger sequencing of amplified PepMV-coat protein gene segments showed ~98% sequence identity to the Chilean (CH2)-strain. In a biological assay testing the contribution of traded infected tomatoes to the establishment of tomato plant disease, we applied direct and indirect inoculation modes using
Identifiants
pubmed: 32422863
pii: plants9050623
doi: 10.3390/plants9050623
pmc: PMC7285381
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Israeli Chief Scientist, Ministry of Agriculture
ID : 20-10-0070 to AD
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