Live-cell protein engineering with an ultra-short split intein.
chemical biology
intein splicing
protein engineering
protein semisynthesis
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
02 06 2020
02 06 2020
Historique:
pubmed:
20
5
2020
medline:
18
8
2020
entrez:
20
5
2020
Statut:
ppublish
Résumé
Split inteins are privileged molecular scaffolds for the chemical modification of proteins. Though efficient for in vitro applications, these polypeptide ligases have not been utilized for the semisynthesis of proteins in live cells. Here, we biochemically and structurally characterize the naturally split intein VidaL. We show that this split intein, which features the shortest known N-terminal fragment, supports rapid and efficient protein
Identifiants
pubmed: 32424098
pii: 2003613117
doi: 10.1073/pnas.2003613117
pmc: PMC7275667
doi:
Banques de données
PDB
['6VGV', '6VGW']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
12041-12049Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM086868
Pays : United States
Organisme : NIGMS NIH HHS
ID : R37 GM086868
Pays : United States
Déclaration de conflit d'intérêts
The authors declare no competing interest.
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