Development of a Novel High-Performance Thin Layer Chromatography-Based Method for the Simultaneous Quantification of Clinically Relevant Lipids from Cells and Tissue Extracts.
Cancer
Cholesterol
Fatty acids
Triacylglycerols
Journal
Lipids
ISSN: 1558-9307
Titre abrégé: Lipids
Pays: United States
ID NLM: 0060450
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
23
09
2019
revised:
10
04
2020
accepted:
23
04
2020
pubmed:
20
5
2020
medline:
12
6
2021
entrez:
20
5
2020
Statut:
ppublish
Résumé
Lipids such as cholesterol, triacylglycerols, and fatty acids play important roles in the regulation of cellular metabolism and cellular signaling pathways and, as a consequence, in the development of various diseases. It is therefore important to understand how their metabolism is regulated to better define the components involved in the development of various human diseases. In the present work, we describe the development and validation of a high-performance thin layer chromatography (HPTLC) method allowing the separation and quantification of free cholesterol, cholesteryl esters, nonesterified fatty acids, and triacylglycerols. This method will be of interest as the quantification of these lipids in one single assay is difficult to perform.
Substances chimiques
Cholesterol Esters
0
Fatty Acids, Nonesterified
0
Lipids
0
Tissue Extracts
0
Triglycerides
0
Cholesterol
97C5T2UQ7J
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
403-412Subventions
Organisme : Institut National de la Santé et de la Recherche Médicale
Pays : International
Organisme : Ligue Contre le Cancer
Pays : International
Organisme : Région Centre-Val de Loire
Pays : International
Organisme : Canceropole Grand-Ouest (Mature project)
Pays : International
Organisme : INCa PLBio
ID : 2018-145
Pays : International
Organisme : University of Tours
Pays : International
Informations de copyright
© 2020 AOCS.
Références
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