Molecular analysis of

Diagnosis FMR1 gene Fragile X syndrome PCR

Journal

Practical laboratory medicine
ISSN: 2352-5517
Titre abrégé: Pract Lab Med
Pays: Netherlands
ID NLM: 101690848

Informations de publication

Date de publication:
Aug 2020
Historique:
received: 23 08 2019
revised: 04 02 2020
accepted: 24 03 2020
entrez: 20 5 2020
pubmed: 20 5 2020
medline: 20 5 2020
Statut: epublish

Résumé

Fragile X syndrome (FXS) is caused by expansion of the number of cytosine-guanine-guanine (CGG) repeats in the regulatory region of the gene and methods: This study had a retrospective design. We analyzed the performance of the FragilEase™ PCR kit using 90 DNA samples from patients with clinical suspicion of FXS or a family history of the syndrome using capillary electrophoresis and compared with the results obtained for the same samples using PCR, SB, and AmplideX FragilEase™ PCR kit displayed high concordance with the results obtained using PCR, SB, and AmplideX FragilEase™ PCR, as well as other commercially available kits, efficiently detect

Identifiants

pubmed: 32426440
doi: 10.1016/j.plabm.2020.e00162
pii: S2352-5517(19)30094-0
pii: e00162
pmc: PMC7225725
doi:

Types de publication

Journal Article

Langues

eng

Pagination

e00162

Informations de copyright

© 2020 The Authors.

Déclaration de conflit d'intérêts

None.

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Auteurs

Cinthia Ramos (C)

Post Graduate Program in Gynecology and Obstetrics, Universidade Federal do Paraná, Curitiba, Brazil.
Department of Genetics, Federal University of Paraná, Curitiba, Brazil.

Maristela Ocampos (M)

Neurogene Laboratory of Human Citogenetics and Molecular Genetics, Florianópolis, Brazil.

Ingrid Tremel Barbato (IT)

Neurogene Laboratory of Human Citogenetics and Molecular Genetics, Florianópolis, Brazil.

Maria da Graça Bicalho (MD)

Post Graduate Program in Gynecology and Obstetrics, Universidade Federal do Paraná, Curitiba, Brazil.
Department of Genetics, Federal University of Paraná, Curitiba, Brazil.

Renato Nisihara (R)

Post Graduate Program in Gynecology and Obstetrics, Universidade Federal do Paraná, Curitiba, Brazil.
Department of Medicine, Positivo University, Curitiba, Paraná, Brazil.

Classifications MeSH