Tissue stiffness contributes to YAP activation in bladder cancer patients undergoing transurethral resection.
Adaptor Proteins, Signal Transducing
/ chemistry
Aged
Biomarkers, Tumor
/ chemistry
Extracellular Matrix
/ metabolism
Female
Humans
Male
Mechanotransduction, Cellular
/ physiology
Middle Aged
Protein Structure, Secondary
Protein Structure, Tertiary
Transcription Factors
/ chemistry
Tumor Microenvironment
/ physiology
Urinary Bladder Neoplasms
/ metabolism
YAP-Signaling Proteins
ECM
Hippo pathway
Yes-associated protein (YAP)
bladder cancer
tissue stiffness
β1 integrin
Journal
Annals of the New York Academy of Sciences
ISSN: 1749-6632
Titre abrégé: Ann N Y Acad Sci
Pays: United States
ID NLM: 7506858
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
18
01
2020
revised:
21
03
2020
accepted:
13
04
2020
pubmed:
20
5
2020
medline:
15
12
2020
entrez:
20
5
2020
Statut:
ppublish
Résumé
Changes in the cellular microenvironment play a critical role in the development of bladder cancer (BC). Yes-associated protein (YAP), a central mediator of the Hippo pathway, functions as a nuclear sensor of mechanotransduction that can be induced by stiffness of the extracellular matrix (ECM), including stiffness resulting from surgical manipulations. We aimed to clarify the possible association between surgically-related ECM stiffness and YAP activation in BC patients. We compared 30 bladder cancer tissues with grade II (n = 15 recurrent and n = 15 newly diagnosed) with 30 adjacent healthy tissues. Atomic force microscopy showed that patients with recurrent BC had stiffer ECM than newly diagnosed patients (P < 0.05). Gene expression profiles showed that β1 integrin (ITGB1), focal adhesion kinase (FAK), CDC42, and YAP were upregulated in cancerous tissues (P < 0.05); additionally, β1 integrin activation was confirmed using a specific antibody. Nuclear localization of YAP was higher in recurrent cancerous tissues compared with newly diagnosed and it was positively associated with higher stiffness (P < 0.05). Our results suggest that postsurgery-induced ECM stiffness can influence integrin-FAK-YAP activity and thereby YAP trafficking to the nucleus where it contributes to BC progression and relapse.
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Biomarkers, Tumor
0
Transcription Factors
0
YAP-Signaling Proteins
0
YAP1 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
48-61Informations de copyright
© 2020 New York Academy of Sciences.
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