Characterization of Stem-like Circulating Tumor Cells in Pancreatic Cancer.

CTC EMT PDAC adherens junctions circulating tumor cells epithelial-mesenchymal transition pancreatic cancer stem cells stem-like stemness

Journal

Diagnostics (Basel, Switzerland)
ISSN: 2075-4418
Titre abrégé: Diagnostics (Basel)
Pays: Switzerland
ID NLM: 101658402

Informations de publication

Date de publication:
15 May 2020
Historique:
received: 17 02 2020
revised: 26 04 2020
accepted: 13 05 2020
entrez: 21 5 2020
pubmed: 21 5 2020
medline: 21 5 2020
Statut: epublish

Résumé

Pancreatic ductal adenocarcinoma (PDAC) is the fourth most frequent cause of death from cancer. Circulating tumor cells (CTCs) with stem-like characteristics lead to distant metastases and thus contribute to the dismal prognosis of PDAC. Our purpose is to investigate the role of stemness in CTCs derived from a genetically engineered mouse model of PDAC and to further explore the potential molecular mechanisms. The publically available RNA sequencing dataset GSE51372 was analyzed, and CTCs with (CTC-S) or without (CTC-N) stem-like features were discriminated based on a principal component analysis (PCA). Differentially expressed genes, weighted gene co-expression network analysis (WGCNA), and further functional enrichment analyses were performed. The prognostic role of the candidate gene (

Identifiants

pubmed: 32429174
pii: diagnostics10050305
doi: 10.3390/diagnostics10050305
pmc: PMC7278018
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : China Scholarship Council
ID : 201908080072

Déclaration de conflit d'intérêts

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

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Auteurs

Lei Zhu (L)

Department of Surgery, Universitätsmedizin Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.

Barbara Hissa (B)

Department of Surgery, Universitätsmedizin Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.

Balázs Győrffy (B)

nd Department of Pediatrics, Semmelweis University, H-1094 Budapest, Hungary.
TTK Cancer Biomarker Research Group, Institute of Enzymology, H-1117 Budapest, Hungary.

Johann-Christoph Jann (JC)

Department of Medicine III, Universitätsmedizin Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.

Cui Yang (C)

Department of Surgery, Universitätsmedizin Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.

Christoph Reissfelder (C)

Department of Surgery, Universitätsmedizin Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
German Cancer Consortium (DKTK) & German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.

Sebastian Schölch (S)

Department of Surgery, Universitätsmedizin Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
German Cancer Consortium (DKTK) & German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.

Classifications MeSH