A novel role for NUAK1 in promoting ovarian cancer metastasis through regulation of fibronectin production in spheroids.
LKB1
NUAK1
fibronectin
metastasis
ovarian cancer
spheroid
Journal
Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829
Informations de publication
Date de publication:
15 May 2020
15 May 2020
Historique:
received:
03
04
2020
revised:
11
05
2020
accepted:
12
05
2020
entrez:
21
5
2020
pubmed:
21
5
2020
medline:
21
5
2020
Statut:
epublish
Résumé
Epithelial ovarian cancer (EOC) has a unique mode of metastasis, where cells shed from the primary tumour, form aggregates called spheroids to evade anoikis, spread through the peritoneal cavity, and adhere to secondary sites. We previously showed that the master kinase Liver kinase B1 (LKB1) is required for EOC spheroid viability and metastasis. We have identified novel (nua) kinase 1 (NUAK1) as a top candidate LKB1 substrate in EOC cells and spheroids using a multiplex inhibitor beads-mass spectrometry approach. We confirmed that LKB1 maintains NUAK1 phosphorylation and promotes its stabilization. We next investigated NUAK1 function in EOC cells. Ectopic NUAK1-overexpressing EOC cell lines had increased adhesion, whereas the reverse was seen in OVCAR8-
Identifiants
pubmed: 32429240
pii: cancers12051250
doi: 10.3390/cancers12051250
pmc: PMC7280971
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : CIHR
ID : 136836
Pays : Canada
Organisme : Genome Canada
ID : OGI139
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