Effect of Paroxetine on the Neuropathic Pain: A Molecular Study.
Animals
Brain-Derived Neurotrophic Factor
/ genetics
Calcium-Binding Proteins
/ metabolism
Gene Expression Regulation
/ drug effects
Male
Microfilament Proteins
/ metabolism
Nerve Tissue
/ drug effects
Neuralgia
/ drug therapy
Paroxetine
/ administration & dosage
Rats, Wistar
Receptors, GABA-A
/ genetics
Symporters
/ genetics
K Cl- Cotransporters
Brain-derived neurotrophic factor
Gamma-aminobutyric acid
Paroxetine
Journal
Iranian biomedical journal
ISSN: 2008-823X
Titre abrégé: Iran Biomed J
Pays: Iran
ID NLM: 9814853
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
entrez:
21
5
2020
pubmed:
21
5
2020
medline:
28
7
2021
Statut:
ppublish
Résumé
Neuropathic pain, due to peripheral nerve damage, has influenced millions of people living all over the world. It has been shown that paroxetine can relieve neuropathic pain. Recently, the role of certain proteins like brain-derived neurotrophic factor (BDNF), GABAA receptor, and K+-Cl- cotransporter 2 (KCC2) transporter in the occurrence of neuropathic pain has been documented. In the current study, the expression of these proteins affected by paroxetine was evaluated. Male Wistar rats were allocated into two main groups of pre- and post-injury. Rats in each main group received paroxetine before nerve injury and at day seven after nerve damage till day 14, respectively. The lumbar spinal cord of animals was extracted to assess the expression of target genes and proteins. In the preventive study, paroxetine decreased BDNF and increased KCC2 and GABAA gene and protein expression, while in the post-injury paradigm, it decreased BDNF and increased KCC2 genes and protein expression. In this regard, an increase in the protein expression of GABAA was observed. It seems that paroxetine with a change in the expression of three significant proteins involved in neuropathic pain could attenuate this type of chronic pain.
Sections du résumé
Background
Neuropathic pain, due to peripheral nerve damage, has influenced millions of people living all over the world. It has been shown that paroxetine can relieve neuropathic pain. Recently, the role of certain proteins like brain-derived neurotrophic factor (BDNF), GABAA receptor, and K+-Cl- cotransporter 2 (KCC2) transporter in the occurrence of neuropathic pain has been documented. In the current study, the expression of these proteins affected by paroxetine was evaluated.
Methods
Male Wistar rats were allocated into two main groups of pre- and post-injury. Rats in each main group received paroxetine before nerve injury and at day seven after nerve damage till day 14, respectively. The lumbar spinal cord of animals was extracted to assess the expression of target genes and proteins.
Results
In the preventive study, paroxetine decreased BDNF and increased KCC2 and GABAA gene and protein expression, while in the post-injury paradigm, it decreased BDNF and increased KCC2 genes and protein expression. In this regard, an increase in the protein expression of GABAA was observed.
Conclusion
It seems that paroxetine with a change in the expression of three significant proteins involved in neuropathic pain could attenuate this type of chronic pain.
Identifiants
pubmed: 32429644
doi: 10.29252/ibj.24.5.301
pmc: PMC7392138
doi:
Substances chimiques
Aif1 protein, rat
0
Brain-Derived Neurotrophic Factor
0
Calcium-Binding Proteins
0
Microfilament Proteins
0
Receptors, GABA-A
0
Symporters
0
Paroxetine
41VRH5220H
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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