Overexpression of Toll-Like Receptor 4 Affects Autophagy, Oxidative Stress, and Inflammatory Responses in Monocytes of Transgenic Sheep.
TLR4
autophagy
inflammatory responses
oxidative stress
p38 MAPK
transgenic animal model
Journal
Frontiers in cell and developmental biology
ISSN: 2296-634X
Titre abrégé: Front Cell Dev Biol
Pays: Switzerland
ID NLM: 101630250
Informations de publication
Date de publication:
2020
2020
Historique:
received:
13
12
2019
accepted:
24
03
2020
entrez:
21
5
2020
pubmed:
21
5
2020
medline:
21
5
2020
Statut:
epublish
Résumé
Toll-like receptor 4 (TLR4) is a critical pattern recognition receptor that plays a critical role in the host innate immune system's recognition of Gram-negative bacteria. Since it is the lipopolysaccharide (LPS) receptor, it links the activated inflammatory response with autophagy and oxidative stress. Autophagy, or type II programmed cell death, was reported to have defensive functions in response to the production of inflammatory cytokines and oxidative stress. To explore the relationship between autophagy, inflammation, and oxidative stress, a TLR4-enriched transgenic (Tg) animal model (sheep) was generated. Autophagy activity in the Tg blood monocytes was significantly higher than in the wild-type animal under LPS stress, and it returned to normal after transfection of TLR4 siRNA. Pretreatment with 3-methyladenine (3-MA) inhibited autophagy and enhanced oxidative stress and the production of TNF-α. The LPS-induced reactive oxygen species (ROS) level was markedly increased in the Tg group at an early stage before quickly returning to normal values. In addition, suppressing ROS production by
Identifiants
pubmed: 32432106
doi: 10.3389/fcell.2020.00248
pmc: PMC7214805
doi:
Types de publication
Journal Article
Langues
eng
Pagination
248Informations de copyright
Copyright © 2020 Wang, Song, Zhang, Deng, Jiao, Qi, Lian and Yao.
Références
J Ethnopharmacol. 2010 Aug 19;131(1):95-103
pubmed: 20558270
Cell. 2010 Mar 19;140(6):805-20
pubmed: 20303872
Neuroscience. 2014 May 16;267:241-51
pubmed: 24607321
Oncotarget. 2015 Nov 24;6(37):39839-54
pubmed: 26497999
J Immunol. 2006 Sep 1;177(5):3273-82
pubmed: 16920968
Oxid Med Cell Longev. 2015;2015:359315
pubmed: 26576220
J Ethnopharmacol. 2013 Jul 9;148(2):544-55
pubmed: 23684722
Nature. 2011 Jan 13;469(7329):221-5
pubmed: 21124315
Sci Rep. 2012;2:896
pubmed: 23193438
Cell. 2016 Feb 25;164(5):896-910
pubmed: 26919428
Biomed Res Int. 2013;2013:568671
pubmed: 23841076
Nat Cell Biol. 2013 Apr;15(4):406-16
pubmed: 23524951
Sci Signal. 2010 May 25;3(123):ra42
pubmed: 20501938
Autophagy. 2018;14(2):190-198
pubmed: 28813180
Cell Death Dis. 2017 Oct 12;8(10):e3113
pubmed: 29022891
J Biol Chem. 2000 Apr 14;275(15):11058-63
pubmed: 10753909
Biochem J. 2007 May 1;403(3):451-61
pubmed: 17217339
Nat Immunol. 2011 Mar;12(3):222-30
pubmed: 21151103
Biochemistry (Mosc). 2004 Jan;69(1):1-3
pubmed: 15127743
Int J Oncol. 2013 Aug;43(2):600-10
pubmed: 23708970
Autophagy. 2016;12(2):245-60
pubmed: 26222012
Annu Rev Pathol. 2009;4:365-98
pubmed: 18928408
J Cell Sci. 2015 Jan 15;128(2):207-17
pubmed: 25568150
Nat Rev Immunol. 2013 Jun;13(6):453-60
pubmed: 23681101
Cardiovasc Res. 2006 Dec 1;72(3):447-55
pubmed: 17064675
Eur J Cell Biol. 2019 Jan;98(1):36-50
pubmed: 30522781
Nat Immunol. 2010 May;11(5):373-84
pubmed: 20404851
Annu Rev Immunol. 2002;20:197-216
pubmed: 11861602
Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6226-31
pubmed: 19339495
PLoS One. 2012;7(10):e47118
pubmed: 23056598
Nat Rev Immunol. 2013 Oct;13(10):722-37
pubmed: 24064518
Int J Biol Sci. 2018 Jun 3;14(9):1022-1032
pubmed: 29989103
J Immunol. 2001 Feb 15;166(4):2651-7
pubmed: 11160328
Cell Physiol Biochem. 2018;49(2):662-677
pubmed: 30165358
Autophagy. 2018;14(11):1850-1869
pubmed: 29985091
Genes Immun. 2006 Jul;7(5):372-83
pubmed: 16738669
Cell Signal. 2000 Jan;12(1):1-13
pubmed: 10676842
Autophagy. 2014 Apr;10(4):699-701
pubmed: 24492492
Cell Metab. 2011 May 4;13(5):495-504
pubmed: 21531332
J Med Microbiol. 2018 Feb;67(2):240-248
pubmed: 29388547
J Oral Pathol Med. 2019 Jan;48(1):87-95
pubmed: 30367515
Int J Mol Sci. 2017 May 18;18(5):
pubmed: 28524116
Am J Cancer Res. 2016 Oct 01;6(10):2162-2177
pubmed: 27822409
Sci Rep. 2015 Dec 11;5:17935
pubmed: 26659006
Immunol Rev. 2012 Mar;246(1):379-400
pubmed: 22435567
J Inflamm (Lond). 2010 Jan 08;7:1
pubmed: 20148136
J Virol. 2001 Nov;75(22):10730-7
pubmed: 11602714
Cell. 2017 Jul 27;170(3):548-563.e16
pubmed: 28753429
Infect Immun. 2004 Apr;72(4):2123-30
pubmed: 15039334
Autophagy. 2014 Mar;10(3):497-513
pubmed: 24384561
Immunol Lett. 2008 Oct 30;120(1-2):57-64
pubmed: 18675302
J Cell Biochem. 2018 Feb;119(2):1604-1615
pubmed: 28771872
Autophagy. 2014 Feb;10(2):257-68
pubmed: 24321786
Cell. 2011 Nov 11;147(4):868-80
pubmed: 22078883