Immunologic timeline of Ebola virus disease and recovery in humans.
Dendritic cells
Immunology
Innate immunity
Monocytes
Virology
Journal
JCI insight
ISSN: 2379-3708
Titre abrégé: JCI Insight
Pays: United States
ID NLM: 101676073
Informations de publication
Date de publication:
21 05 2020
21 05 2020
Historique:
received:
17
02
2020
accepted:
22
04
2020
entrez:
22
5
2020
pubmed:
22
5
2020
medline:
19
5
2021
Statut:
epublish
Résumé
A complete understanding of human immune responses to Ebola virus infection is limited by the availability of specimens and the requirement for biosafety level 4 (BSL-4) containment. In an effort to bridge this gap, we evaluated cryopreserved PBMCs from 4 patients who survived Ebola virus disease (EVD) using an established mass cytometry antibody panel to characterize various cell populations during both the acute and convalescent phases. Acute loss of nonclassical monocytes and myeloid DCs, especially CD1c+ DCs, was noted. Classical monocyte proliferation and CD38 upregulation on plasmacytoid DCs coincided with declining viral load. Unsupervised analysis of cell abundance demonstrated acute declines in monocytic, NK, and T cell populations, but some populations, many of myeloid origin, increased in abundance during the acute phase, suggesting emergency hematopoiesis. Despite cell losses during the acute phase, upregulation of Ki-67 correlated with recovery of cell populations over time. These data provide insights into the human immune response during EVD.
Identifiants
pubmed: 32434986
pii: 137260
doi: 10.1172/jci.insight.137260
pmc: PMC7259516
doi:
pii:
Substances chimiques
Ki-67 Antigen
0
MKI67 protein, human
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : K08 AI119448
Pays : United States
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