Molecular Identification, Characterization, and Expression Analysis of a Metallothionein Gene from Septifer virgatus.
Biomarker
Heavy metal
Metallothionein
Septifer virgatus
Journal
Marine biotechnology (New York, N.Y.)
ISSN: 1436-2236
Titre abrégé: Mar Biotechnol (NY)
Pays: United States
ID NLM: 100892712
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
22
01
2020
accepted:
03
04
2020
pubmed:
22
5
2020
medline:
7
4
2021
entrez:
22
5
2020
Statut:
ppublish
Résumé
This study provides a preliminary characterization of a metallothionein (MT) gene in Septifer virgatus and highlights its potential use in biomonitoring. The full-length SvMT cDNA and the complete sequence of the SvMT gene were identified using reverse transcriptase PCR coupled with the rapid amplification of cDNA ends and the primer walking method. The SvMT cDNA encodes a protein of 72 amino acids having nine classical Cys-X-Cys motifs. Moreover, the deduced amino acids contained the conserved motif (Cys-x-Cys-x(3)-Cys-Thr-Gly-x(3)-Cys-x-Cys-x(3)-Cys-x-Cys-Lys) of MT family 2. Its molecular mass and isoelectric point were estimated to be 7.01 kDa and 7.00, respectively. BLAST-based searching indicated that SvMT shared 81.0% amino acid sequence identity with Mytilus edulis MT-20-II. The SvMT gene has three coding exons and two introns. After exposure to 1 mg/L cadmium chloride, the expression of SvMT increased 15-fold by 3 days (d), with a maximum expression of 27-fold by 5 d compared with the pre-exposure level. After exposure to 2 mg/L zinc chloride, the expression of SvMT increased 2.5-fold by 3 d and 4.7-fold by 5 d compared with the pre-exposure level. A significant increase in the expression level of SvMT mRNA was observed after the exposure of S. virgatus to the combination of 0.003 mg/L cadmium chloride and 0.2 mg/L zinc chloride compared with the pre-exposure level. Our work indicates that the SvMT gene is associated with stress responses and could be a potential biomarker for marine pollution.
Identifiants
pubmed: 32435938
doi: 10.1007/s10126-020-09970-7
pii: 10.1007/s10126-020-09970-7
doi:
Substances chimiques
Chlorides
0
DNA, Complementary
0
Environmental Biomarkers
0
Zinc Compounds
0
zinc chloride
86Q357L16B
Metallothionein
9038-94-2
Cadmium Chloride
J6K4F9V3BA
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
488-497Références
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